Extreme heterogeneity of influenza virus infection in single cells
Extreme heterogeneity of influenza virus infection in single cells
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DOI：
10.7554/elife.32303
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发表时间：
2018-02-16
期刊：
影响因子：
7.7
通讯作者：
Bloom, Jesse D.
中科院分区：
文献类型：
--
作者：
Russell, Alistair B.;Trapnell, Cole;Bloom, Jesse D.
病毒感染可以显著改变细胞的转录组。然而，这些变化主要是通过对许多细胞的大量测量来研究的。在这里，我们使用单细胞mRNA测序来检查流感病毒感染的转录后果。我们发现病毒转录的生产力在细胞与细胞之间存在非常广泛的差异-在许多感染的细胞中，病毒转录物占总mRNA的比例不到1%，但少数细胞的mRNA超过一半来自病毒。一些受感染的细胞不能表达至少一个病毒基因，但这种基因的缺失只能部分解释病毒转录载量的变化。尽管病毒载量不同，但病毒mRNA的相对丰度在感染细胞中相当一致。先天免疫途径的激活是罕见的，但一些细胞基因与病毒mRNA的量共同变化。总的来说，我们的研究结果突出了病毒感染在单细胞水平上的复杂性。
Viral infection can dramatically alter a cells transcriptome. However, these changes have mostly been studied by bulk measurements on many cells. Here we use single-cell mRNA sequencing to examine the transcriptional consequences of influenza virus infection. We find extremely wide cell-to-cell variation in the productivity of viral transcription - viral transcripts comprise less than a percent of total mRNA in many infected cells, but a few cells derive over half their mRNA from virus. Some infected cells fail to express at least one viral gene, but this gene absence only partially explains variation in viral transcriptional load. Despite variation in viral load, the relative abundances of viral mRNAs are fairly consistent across infected cells. Activation of innate immune pathways is rare, but some cellular genes co-vary in abundance with the amount of viral mRNA. Overall, our results highlight the complexity of viral infection at the level of single cells.