Cold preservation of rat osteochondral tissues in two types of solid organ preservation solution, culture medium and saline
Cold preservation of rat osteochondral tissues in two types of solid organ preservation solution, culture medium and saline
复制标题
两种固体器官保存液、培养基和生理盐水中大鼠骨软骨组织的冷保存
作者：
Kenji Onuma;K. Urabe;Kouji Naruse;H. J. Park;K. Uchida;M. Itoman
我们比较了 Dulbecco 改良 Eagle’s 培养基 (DMEM)、盐水、欧洲柯林斯 (EC) 溶液和威斯康星大学 (UW) 溶液，以确定哪一种最适合冷藏保存大鼠骨软骨组织 (OCT)。冷藏 7 天后，四唑测定显示，保存在 UW 溶液中的 OCT 具有最高的相对活细胞数，并且受损细胞释放的乳酸脱氢酶活性最低。组织学评估显示软骨细胞畸形，例如细胞质萎缩和核固缩，特别是在盐水和 EC 溶液中保存后关节软骨的深层，如果保存在 DMEM 中则主要在所有层中。相比之下，保存在 UW 溶液中的关节软骨各层的软骨细胞形态相对没有变化，与新鲜的 OCT 保持相似。因此得出结论，UW溶液最适合大鼠OCT以及实体器官的冷藏。
We compared Dulbecco’s modified Eagle’s medium (DMEM), saline, Euro-Collins (EC) solution and University of Wisconsin (UW) solution to determine which was best for cold preservation of rat osteochondral tissues (OCTs). After 7 days’ cold preservation, OCTs kept in UW solution had the highest relative viable cell number by the tetrazolium assay and the lowest activity of lactate dehydrogenase released from damaged cells. Histological evaluation revealed chondrocyte deformity, such as shrunken cytoplasm and pyknotic nuclei, particularly in the deeper layer of articular cartilage after preservation in saline and EC solution and predominantly in all layers if preserved in DMEM. In contrast, chondrocyte morphology in all layers of the articular cartilage preserved in UW solution was relatively unchanged and remained similar to fresh OCTs. It is therefore concluded that UW solution is the most suitable for cold preservation of rat OCTs as well as solid organs.
DOI：
10.2106/00004623-200311000-00008
发表时间：
2003-11-01
影响因子：
5.3
作者：
Williams, SK;Amiel, D;Bugbee, WD
通讯作者：
Bugbee, WD