MICRORNA-29A/PTEN PATHWAY MODULATES NEURITE OUTGROWTH IN PC12 CELLS
MICRORNA-29A/PTEN PATHWAY MODULATES NEURITE OUTGROWTH IN PC12 CELLS
复制标题
MicroRNA-29A/PTEN 通路调节 PC12 细胞中的神经突生长
DOI：
10.1016/j.neuroscience.2015.01.055
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发表时间：
2015-04-16
期刊：
影响因子：
3.3
通讯作者：
Gong, A.
中科院分区：
文献类型：
--
作者：
Zou, H.;Ding, Y.;Gong, A.
PTEN serves as an intrinsic brake on neurite outgrowth, but the regulatory mechanism that governs its action is not clear. In the present study, miR-29a was found to increase neurite outgrowth by decreasing PTEN expression. Results showed that miR-92a-1, miR-29a, miR-92b, and miR-29c expression levels increased during nerve growth factor (NGF)-induced differentiation of PC12 cells. Based on in silico analysis of possible miR-29a targets, PTEN mRNA may be a binding site for miR-29a. A protein expression assay and luciferase reporter assay showed that miR-29a could directly target the 3'-UTRs (untranslated regions) of PTEN mRNA and down-regulate the expression of PTEN. PC12 cells infected with lentiviral pLKO-miR-29a showed far higher levels of miR-29a and Akt phosphorylation level than those infected with control. This promoted neurite outgrowth of PC12 cells. Collectively, these results indicate that miR-29a is an important regulator of neurite outgrowth via targeting PTEN and that it may be a promising therapeutic target for neural disease. (C) 2015 IBRO. Published by Elsevier Ltd. All rights reserved.
