Mode of action of plectasin-derived peptides against gas gangrene-associated Clostridium perfringens type A.
Mode of action of plectasin-derived peptides against gas gangrene-associated Clostridium perfringens type A.
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菌丝菌素衍生肽对抗气性坏疽相关的 A 型产气荚膜梭菌的作用方式
DOI：
10.1371/journal.pone.0185215
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Zheng X;Wang X;Teng D;Mao R;Hao Y;Yang N;Zong L;Wang J
NZ2114 and MP1102 are novel plectasin-derived peptides with potent activity against Gram-positive bacteria. The antibacterial characteristics and mechanism of NZ2114 and MP1102 against gas gangrene-associated Clostridium perfringens were studied for the first time. The minimal inhibitory concentration and minimal bactericidal concentration of NZ2114 and MP1102 against resistant C. perfringens type A strain CVCC 46 were 0.91 μM. Based on the fractional inhibitory concentration index (FICI) result, an additive or synergic effect was observed between NZ2114 (FICI = 0.5~0.75) or MP1102 (FICI = 0.375~1.0) and antibiotics. The flow cytometry, scanning and transmission electron microscopy analysis showed that both NZ2114 and MP1102 induced obviously membrane damage, such as the leakage of cellular materials, partial disappearance of the cell membrane and membrane peeling, as well as retracting cytoplasm and ghost cell. The gel retardation and circular dichroism (CD) detection showed that NZ2114 and MP1102 could bind to C. perfringens genomic DNA and change the DNA conformation. Moreover, NZ2114 also interfered with the double helix and unwind the genomic DNA. The cell cycle analysis showed that C. perfringens CVCC 46 cells exposed to NZ2114 and MP1102 were arrested at the phase I. These data indicated that both NZ2114 and MP1102 have potential as new antimicrobial agents for gas gangrene infection resulting from resistant C. perfringens.
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17 残基牛乳铁蛋白杀死金黄色葡萄球菌和肠炎沙门氏菌并中和脂多糖：提高含 Trp/Ala 分子的活性
影响因子：
4.6
作者：
Hao Y;Yang N;Wang X;Teng D;Mao R;Wang X;Li Z;Wang J
通讯作者：
Wang J
DOI：
10.1016/j.ijantimicag.2005.08.010
发表时间：
2005-11-01
影响因子：
10.8
作者：
Kuwano, K;Tanaka, N;Sonomoto, K
通讯作者：
Sonomoto, K
影响因子：
64.8
作者：
Mygind, PH;Fischer, RL;Kristensen, HH
通讯作者：
Kristensen, HH
影响因子：
4.4
作者：
Jiao, Jian;Mao, Ruoyu;Wang, Jianhua
通讯作者：
Wang, Jianhua
DOI：
10.1128/aem.20.4.633-637.1970
发表时间：
1970-01-01
期刊：
APPLIED MICROBIOLOGY
影响因子：
--
作者：
ARANYI, C;FENTERS, J;TOLKACZ, V
通讯作者：
TOLKACZ, V