Hepatic mixed function oxidase activity in rapidly prepared microsomes
Hepatic mixed function oxidase activity in rapidly prepared microsomes
复制标题
快速制备的微粒体中的肝混合功能氧化酶活性
DOI：
10.1016/0024-3205(72)90020-3
复制
发表时间：
1972
期刊：
影响因子：
6.1
通讯作者：
D. L. Cinti
中科院分区：
文献类型：
--
作者：
J. Schenkman;D. L. Cinti
本文对一种新的快速分离肝微粒体的方法进行了评价。新制备从肝脏分离到微粒体悬液工作需要大约1小时。通过这种Ca+2辅助沉降获得的微粒体在药物代谢活性或血红素蛋白含量方面与常规制备的微粒体没有差异。然而，在形态学上，在Ca+2存在下制备的微粒体失去了它们的核糖体，这些核糖体聚集。
A new method for rapid isolation of hepatic microsomes has been evaluated with respect to the drug biotransformation system. The new preparation requires approximately one hour from the isolation of liver to a working microsomal suspension. The microsomes obtained by this Ca+2-aided sedimentation did not differ in drug metabolizing activity or hemoprotein content from the conventionally prepared microsomes. Morphologically, however, the microsomes prepared in the presence of Ca+2lose their ribosomes, which aggregate.