Human choriogonadotropin-like material in bacteria of different species: electron microscopy and immunocytochemical studies with monoclonal and polyclonal antibodies.
Human choriogonadotropin-like material in bacteria of different species: electron microscopy and immunocytochemical studies with monoclonal and polyclonal antibodies.
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不同物种细菌中的人绒毛膜促性腺激素样物质：电子显微镜和单克隆和多克隆抗体的免疫细胞化学研究。
DOI：
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
G. Domingue
中科院分区：
文献类型：
--
作者：
H. Acevedo;M. Pardo;E. Campbell‐Acevedo;G. Domingue
免疫细胞化学研究，使用抗血清，以整个人绒毛膜促性腺激素（hCG），其α-和β-亚基和COOH-末端肽的hCG β，和两个单克隆抗体hCG β，表现出表达的hCG样物质，其个别亚基和/或片段在9个菌株。其中7个是从癌症患者中分离出来的，明确鉴定为粪链球菌（3株）、溶血葡萄球菌（2株）和表皮葡萄球菌和大肠杆菌（单一菌株）。另外两种菌株是细胞壁缺陷（CWD）变体，其中一种被鉴定为牛链球菌，从一名不明原因发热和可能脑脓肿的患者血液中分离出来。另一种是从孕妇尿液中分离的革兰氏阴性类白喉杆菌，在研究期间，它恢复为革兰氏阳性棒状杆菌，被鉴定为“C”。在革兰氏阴性类白喉时表达hCG样因子。这9个菌株的电子显微镜检查（包括相同种属菌株的阴性对照，进行相同的免疫细胞化学分析，并在相同的培养条件下）显示细菌细胞壁和细胞质物质的形态学改变和/或在9个表达hCG样物质（包括两种CWD变体）的菌株中的6个菌株中的奇异繁殖形式。总的来说，这些结果提供了证据：（1）从明显的癌症患者中分离出的产生hCG的细菌并不是其他人声称的新的和独特的物种，（2）基于hCG分子不同部分的免疫化学识别，细菌蛋白和人类滋养层激素之间存在密切的相似性。（250字处删节）
Immunocytochemical studies using antisera to whole human choriogonadotropin (hCG), to its alpha- and beta-subunits and to the COOH-terminal peptide of hCG beta, and two monoclonal antibodies to hCG beta, demonstrated expression of hCG-like material, its individual subunits and/or fragments in nine bacterial strains. Seven of these were isolated from patients with cancer and were definitely identified as Streptococcus faecalis (three strains), Staphylococcus haemolyticus (two strains) and Staphylococcus epidermidis and Escherichia coli (single strains). The other two strains were cell-wall-deficient (CWD) variants, one identified as Streptococcus bovis, isolated from the blood of a patient with a fever of unknown origin and a possible brain abscess. The other was a Gram-negative diphtheroid isolated from the urine of a pregnant woman, which during the period of study reverted to a Gram-positive Corynebacterium identified as a 'C. ulcerans' strain and expressed the hCG-like factor only during its phase as Gram-negative diphtheroid. Electron microscopy of these nine strains (including negative controls of strains of the same species subjected to the same immunocytochemical analyses and under identical cultural conditions) revealed morphological alterations in the bacterial cell walls and cytoplasmic material and/or bizarre forms of reproduction in six of the nine strains expressing hCG-like material including the two CWD variants. Collectively, these results provided evidence that (1) hCG-producing bacteria isolated from patients with overt cancer are not a new and unique species as claimed by others, and (2) there is a close resemblance between the bacterial protein and the human trophoblastic hormone, based on immunochemical recognition of different parts of the hCG molecule.(ABSTRACT TRUNCATED AT 250 WORDS)
人绒毛膜促性腺激素的生物合成。
影响因子：
20.3
作者：
Hussa,RO
通讯作者：
Hussa,RO