ISOLATION OF CORONAVIRUSES ANTIGENICALLY INDISTINGUISHABLE FROM BOVINE CORONAVIRUS FROM WILD RUMINANTS WITH DIARRHEA
ISOLATION OF CORONAVIRUSES ANTIGENICALLY INDISTINGUISHABLE FROM BOVINE CORONAVIRUS FROM WILD RUMINANTS WITH DIARRHEA
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DOI：
10.1128/jcm.33.12.3264-3269.1995
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发表时间：
1995-12-01
影响因子：
9.4
通讯作者：
SAIF, LJ
中科院分区：
文献类型：
--
作者：
TSUNEMITSU, H;ELKANAWATI, ZR;SAIF, LJ
在俄亥俄州野生动物养殖场采集的3只水鹿和1只水羚以及1只白尾鹿的腹泻粪便中均含有冠状病毒颗粒，免疫电镜观察发现这些颗粒与牛冠状病毒抗血清凝集。从水鹿、白尾鹿和水羚粪便中分离到三株冠状病毒，分别进行了血凝、受体破坏酶活性、间接免疫荧光、血凝抑制、病毒中和和Western印迹试验（免疫印迹）试验显示分离株之间以及与选定的BCV菌株之间存在密切的生物学和抗原关系，接种了这些分离株中的每一种的无菌和初乳剥夺小牛发生腹泻，并在其粪便和鼻道中排出冠状病毒。在野鹿中冠状病毒感染的血清学调查中，来自怀俄明州的黑尾鹿和来自俄亥俄州的白尾鹿的血清中分别有8.7%和6.6%，间接免疫荧光试验结果表明，野生反刍动物中存在冠状病毒，并提示这些动物可能携带有可传牛的冠状病毒株。
Diarrheal feces from three sambar deer and one waterbuck in a wild animal habitat and one white-tailed deer on a wildlife farm in Ohio contained coronavirus particles which were agglutinated by antiserum to bovine coronavirus (BCV) in immune electron microscopy. Three coronavirus strains were isolated in human rectal tumor cells from the feces of the sambar and white-tailed deer and the waterbuck, respectively, Hemagglutination, receptor-destroying enzyme activity, indirect immunofluorescence, hemagglutination inhibition, virus neutralization, and Western blot (immunoblot) tests showed close biological and antigenic relationships among the isolates and with selected BCV strains, Gnotobiotic and colostrum deprived calves inoculated with each of these isolates developed diarrhea and shed coronavirus in their feces and from their nasal passages, In a serological survey of coronavirus infections among wild deer, 8.7 and 6.6% of sera from mule deer in Wyoming and from white-tailed deer in Ohio, respectively, were seropositive against both of the isolates and selected BCV isolates by indirect immunofluorescence tests, These results confirm the existence of coronaviruses in wild ruminants and suggest that these species may harbor coronavirus strains transmissible to cattle.