Expression analysis of brain-derived neurotrophic factor (BDNF) mRNA isoforms after chronic and acute antidepressant treatment
Expression analysis of brain-derived neurotrophic factor (BDNF) mRNA isoforms after chronic and acute antidepressant treatment
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DOI：
10.1016/j.brainres.2003.12.028
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发表时间：
2004-03-12
期刊：
影响因子：
2.9
通讯作者：
Jansson, BO
中科院分区：
文献类型：
--
作者：
Altieri, M;Marini, F;Jansson, BO
脑源性神经营养因子（BDNF）被认为是神经元存活、分化和可塑性的关键因子。根据提出的假设模型，BDNF的表达可能在抑郁症的发病机制中发挥核心作用。BDNF基因的结构相当复杂，它可以通过选择性剪接表达四种不同的mRNA亚型，每种亚型产生相同的蛋白质。这可能反映了不同信号网络对基因调控的微调。由于BDNF基因已被报道被抗抑郁药上调，四个BDNF mRNA亚型的表达进行了测量，通过实时定量RT-PCR在大鼠海马慢性和急性治疗后的抗抑郁药氟西汀和GR 205171，一种选择性的NK-1受体拮抗剂与抗焦虑的属性。本研究的目的是检验这些化合物对mRNA同种型的差异调节的假设。我们的研究结果表明，BDNF mRNA亚型的表达不受氟西汀或GR 205171的慢性或急性治疗的影响。(C)2004 Elsevier B. V.保留所有权利。
The neurotrophin brain-derived neurotrophic factor (BDNF) is considered to be a key factor for neuronal survival, differentiation and plasticity. According to a proposed hypothetical model BDNF expression might play a central role in the pathogenesis of depression. The BDNF gene is rather complex in its structure and it can express four different mRNA isoforms by alternative splicing, each producing the same protein. This might reflect fine tuning of gene regulation by different signalling networks. Since the BDNF gene has been reported to be upregulated by antidepressants, the expression of the four BDNF mRNA isoforms was measured by real-time quantitative RT-PCR in rat hippocampi after chronic and acute treatment with the antidepressant drug fluoxetine and GR205171, a selective NK-1 receptor antagonist with anxiolytic-like properties. The aim of this Study was to test the hypothesis of differential regulation of the mRNA isoforms by those Compounds. Our results indicate that the expression of BDNF mRNA isoforms is not affected by chronic or acute treatment with fluoxetine or GR205171. (C) 2004 Elsevier B.V. All rights reserved.