Immunochemical detection of inducible NO synthase in human lung.
Immunochemical detection of inducible NO synthase in human lung.
复制标题
人肺中诱导型 NO 合酶的免疫化学检测。
DOI：
10.1152/ajplung.1994.266.6.l722
复制
发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Johns,RA
中科院分区：
文献类型：
--
作者：
Tracey,WR;Xue,C;Klinghofer,V;Barlow,J;Pollock,JS;Forstermann,U;Johns,RA
Type II (inducible) nitric oxide synthase (NOS) may play an important role in pulmonary pathophysiology, yet it remains controversial whether human tissues are capable of expressing this protein. Therefore, a polyclonal antibody (8196) was raised against type II NOS from induced RAW 264.7 macrophages and used to investigate the expression of this enzyme in human lung tissue. Anti-type II NOS antibody did not cross-react with either neuronal (type I) or endothelial (type III) constitutive NOS, whereas a 130-kDa protein was detected in cytosol from induced macrophages or liver removed from lipopolysaccharide (25 mg/kg)-treated rats. Cells or tissues that lacked NOS activity did not express immunoreactive proteins. Similarly, in grossly normal human lung tissue, no immunoreactivity was detected with the anti-type II NOS antibody. In contrast, strong immunoreactivity was detected in alveolar macrophages present in lung tissue from a patient with bronchiectasis and acute bronchopneumonia. These data demonstrate that human alveolar macrophages are able to express type II NOS and support a role for this enzyme in pulmonary inflammatory pathophysiology.
