Postnatal maturation of mossy fibre excitatory transmission in mouse CA3 pyramidal cells: a potential role for kainate receptors
Postnatal maturation of mossy fibre excitatory transmission in mouse CA3 pyramidal cells: a potential role for kainate receptors
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DOI：
10.1113/jphysiol.2004.069922
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发表时间：
2004-11-15
影响因子：
5.5
通讯作者：
Mulle, C
中科院分区：
文献类型：
--
作者：
Marchal, C;Mulle, C
红藻氨酸受体（KARs）在中枢神经系统中大量表达，处于突触形成的高峰期，可能参与神经网络的成熟。我们已经描述了生后发育的苔藓纤维兴奋性突触传递CA 3锥体细胞，我们已经探讨了潜在的作用，KARs在突触成熟。在CA 3锥体细胞中，苔藓纤维刺激早在出生后第3天（P3）就诱发EPSC。在这个早期阶段，苔藓纤维（MF）-EPSC完全阻断GYKI 53655，AMPA受体（AMPAR）拮抗剂。突触后KAR成分只能从P6检测到。因此，AMPAR-EPSC先于KAR-EPSC在出生后成熟在这个突触。所有MF-EPSC在PIO后显示KAR组件。目前工作的一个关键问题是，在P6和P9之间，突触后KAR成分的存在与AMPAR介导的大幅度EPSC紧密一致，并且与低频易化（从0.1 Hz到1 Hz）的开始一致，这是一种突触前形式的短期突触可塑性。此外，由于突触传递的突触前和突触后损伤，在整个出生后发育过程中缺乏功能性KAR的小鼠在突触KAR正常存在的成熟阶段显示出幅度显著较小的MF-EPSC。这些数据表明，在苔藓纤维突触的成熟KAR的作用。
Kainate receptors (KARs) are abundantly expressed in the central nervous system at a period of intense synaptogenesis and might participate in the maturation of neural networks. We have described the postnatal development of mossy fibre excitatory synaptic transmission in CA3 pyramidal cells and we have explored the potential role of KARs in synaptic maturation. In CA3 pyramidal cells, mossy fibre stimulation evokes EPSCs as early as postnatal day 3 (P3). At this early stage, mossy fibre (MF)-EPSCs are fully blocked by GYKI 53655, an AMPA receptor (AMPAR) antagonist. A postsynaptic KAR component can only be detected from P6. Thus, AMPAR-EPSCs precede KAR-EPSCs during postnatal maturation at this synapse. All MF-EPSCs display a KAR component after PIO. A key issue of the present work is that between P6 and P9, the presence of a postsynaptic KAR component tightly coincides with AMPAR-mediated EPSCs of large amplitude, and with the onset of low frequency facilitation (from 0.1 Hz to 1 Hz), a presynaptic form of short-term synaptic plasticity. In addition, mice lacking functional KARs throughout postnatal development display MF-EPSCs of significantly smaller amplitude at stages of maturation where synaptic KARs are normally present, due to both pre- and postsynaptic impairment of synaptic transmission. These data suggest a role for KARs in the maturation of mossy fibre synapses.