Shotgun Proteomics Identifies Active Metabolic Pathways in Bleached Coral Tissue and Intraskeletal Compartments
Shotgun Proteomics Identifies Active Metabolic Pathways in Bleached Coral Tissue and Intraskeletal Compartments
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DOI：
10.3389/fmars.2022.797517
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发表时间：
2022-02-14
影响因子：
3.7
通讯作者：
Padilla-Gamino, Jacqueline L.
中科院分区：
文献类型：
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作者：
Axworthy, Jeremy B.;Timmins-Schiffman, Emma;Padilla-Gamino, Jacqueline L.
珊瑚白化事件的频率和强度越来越高，世界上许多造礁珊瑚都处于危险之中。一些珊瑚在漂白后似乎更有弹性，但它们从漂白中恢复并持续存在的能力的机制尚未完全了解。我们使用鸟枪蛋白质组学来比较实验漂白和对照（即，非漂白）的Montipora capitata的殖民地，Montipora capitata是一种夏威夷的物种，以其漂白后的恢复力而闻名。我们在漂白和非漂白个体的OL和IC隔室中鉴定了2，361种蛋白质。在漂白珊瑚的OL中，63种蛋白质显着更丰富，28种蛋白质显着不丰富，而非漂白珊瑚的OL。在IC的漂白珊瑚，22种蛋白质显着更丰富，17个显着不丰富的IC相比，非漂白珊瑚。基因本体论（GO）和途径分析揭示了发生在漂白珊瑚，但不是在非漂白珊瑚的代谢过程。漂白珊瑚的OL使用乙醛酸循环从内部储存化合物（如脂质）中获得碳，具有高蛋白质周转率，并将对氮的依赖从氨转移到尿素和甜菜碱分解产生的氮。漂白珊瑚的IC将葡萄糖分流到戊糖磷酸途径。漂白珊瑚增加了丰度的几种抗氧化剂蛋白质在OL和IC车厢相比，非漂白珊瑚。这些结果突出了漂白M的不同隔室响应漂白胁迫的对比策略。capitata和揭示漂白恢复力背后的一些潜在机制。
Coral bleaching events are increasing with such frequency and intensity that many of the world's reef-building corals are in peril. Some corals appear to be more resilient after bleaching but the mechanisms underlying their ability to recover from bleaching and persist are not fully understood. We used shotgun proteomics to compare the proteomes of the outer layer (OL) tissue and inner core (IC) tissue and skeleton compartments of experimentally bleached and control (i.e., non-bleached) colonies of Montipora capitata, a perforate Hawaiian species noted for its resilience after bleaching. We identified 2,361 proteins in the OL and IC compartments for both bleached and non-bleached individuals. In the OL of bleached corals, 63 proteins were significantly more abundant and 28 were significantly less abundant compared to the OL of non-bleached corals. In the IC of bleached corals, 22 proteins were significantly more abundant and 17 were significantly less abundant compared to the IC of non-bleached corals. Gene ontology (GO) and pathway analyses revealed metabolic processes that were occurring in bleached corals but not in non-bleached corals. The OL of bleached corals used the glyoxylate cycle to derive carbon from internal storage compounds such as lipids, had a high protein turnover rate, and shifted reliance on nitrogen from ammonia to nitrogen produced from the breakdown of urea and betaine. The IC of bleached corals compartmentalized the shunting of glucose to the pentose phosphate pathway. Bleached corals increased abundances of several antioxidant proteins in both the OL and IC compartments compared to non-bleached corals. These results highlight contrasting strategies for responding to bleaching stress in different compartments of bleached M. capitata and shed light on some potential mechanisms behind bleaching resilience.