Applied Electric Fields Polarize Initiation and Growth of Endothelial Sprouts
Applied Electric Fields Polarize Initiation and Growth of Endothelial Sprouts
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DOI：
10.1155/2023/6331148
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发表时间：
2023-12-23
影响因子：
3.3
通讯作者：
Messerli，Mark A.
中科院分区：
文献类型：
--
作者：
Sarkar，Anyesha;Messerli，Shanta M.;Messerli，Mark A.
将治疗电场（EF）应用于表皮，以加速慢性表皮伤口的愈合并促进皮肤移植。虽然研究强调了解 EF 在极化浅表表皮细胞迁移中的作用，但没有报告描述 EF 对底层脉管系统极化的影响。我们探讨了 EF 对内皮芽生长的影响，内皮芽是功能性血管的前体。我们发现，受伤表皮附近具有相同强度的直流电电场会极化内皮芽的起始、生长和转向阳极。虽然 EF 使芽极化，但它们不会改变初生芽或分枝形成的频率。单向电脉冲还根据豆芽的时间平均 EF 幅度来极化它们。发芽极化与电驱动水流（电渗透）的方向反平行，并且与压力驱动水流引起的发芽极化方向一致。这些结果支持 EF 在软组织和组织工程愈合过程中控制新血管形成方向的作用。
Therapeutic electric fields (EFs) are applied to the epidermis to accelerate the healing of chronic epidermal wounds and promote skin transplantation. While research has emphasized understanding the role of EFs in polarizing the migration of superficial epidermal cells, there are no reports describing the effect of EFs on polarization of the underlying vasculature. We explored the effects of EFs on the growth of endothelial sprouts, precursors to functional blood vessels. We discovered that DC EFs of the same magnitude near wounded epidermis polarize initiation, growth, and turning of endothelial sprouts toward the anode. While EFs polarize sprouts, they do not change the frequency of primary sprout or branch formation. Unidirectional electrical pulses also polarize sprouts based on their time‐averaged EF magnitude. Sprout polarization occurs antiparallel to the direction of electrically driven water flow (electro‐osmosis) and is consistent with the direction of sprout polarization induced by pressure‐driven flow. These results support the role of EFs in controlling the direction of neovascularization during the healing of soft tissues and tissue engineering.