Biomechanical response of arterial wall to DOCA-salt hypertension in growing and middle-aged rats
Biomechanical response of arterial wall to DOCA-salt hypertension in growing and middle-aged rats
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DOI：
10.1016/j.jbiomech.2006.07.021
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发表时间：
2007-01-01
影响因子：
2.4
通讯作者：
Sugimoto, Takanori
中科院分区：
文献类型：
--
作者：
Hayashi, Kozaburo;Sugimoto, Takanori
为研究高血压对动脉重塑的影响，建立幼年(16周)和中年(48周)大鼠脱氧皮质酮(DOCA)-盐性高血压模型，测定颈总动脉生物力学特性和管壁尺寸。幼龄大鼠于术后10周或16周切除动脉段，中年大鼠于16周切除动脉段。体外压力-直径测试在正常(在Krebs-Ringer溶液中)、主动(去甲肾上腺素)和被动(罂粟碱)条件下进行。未治疗、年龄匹配的大鼠(26周、32周和周)用于获得对照数据。所有年龄段的高血压患者体内血压水平时的管壁厚度均增加，但内径无明显变化。高血压大鼠正常状态下动脉外径小于被动状态下的动脉外径，提示高血压大鼠血管平滑肌张力增加。高血压使去甲肾上腺素引起的内径缩小明显增加，在100毫米汞柱以上有显著差异；然而，如果分别在体内血压下比较，高血压和正常血压动脉之间没有显著差异。在活体压力下，没有观察到显著的壁硬度差异。26周龄动脉在体血压时的壁环应力与血压呈显著正相关。然而，在32周龄和周龄的动脉中，高血压和正常血压之间的应激没有差异。这些结果基本上与先前在Goldblatt高血压和年轻动物身上观察到的结果相似。动脉壁重构的年龄差异不明显。(C)2006爱思唯尔有限公司。保留所有权利。
To study arterial remodeling in response to hypertension, Deoxycortico-sterone acetate (DOCA)-salt hypertension was induced in immature (aged 16 weeks) and middle-aged (48 weeks) rats, and biomechanical properties and wall dimensions of common carotid arteries were determined. Arterial segments were excised at 10 or 16 weeks postoperatively from the immature rats and at 16 weeks from the middle-aged ones. In vitro pressure-diameter tests were performed under normal (in Krebs-Ringer solution), active (norepinephrine), and passive (papaverine) conditions. Non-treated, age-matched rats (26, 32, and 64 weeks) were used to obtain control data. Wall thickness at in vivo blood pressure level was increased by hypertension at all ages; however, there were no significant changes in inner diameter. In hypertensive rats, arterial outer diameter was smaller under normal condition than under passive condition, indicating the increase of smooth muscle tone by hypertension. Diameter reduction developed by norepinephrine was increased by hypertension, which was significant above 100 mmHg; however, there were no significant differences between hypertensive and normotensive arteries, if compared at respective in vivo blood pressures. No significant differences were observed in wall stiffness at in vivo pressure. Wall hoop stress at in vivo blood pressure had a significant positive correlation with the pressure in 26-week old arteries. However, there were no differences in the stress between hypertension and normotension in 32- and 64-week old arteries. These results were essentially similar to previous ones observed in Goldblatt hypertension and in younger animals. Age-related differences in arterial wall remodeling were not clearly observed. (C) 2006 Elsevier Ltd. All rights reserved.