Functional and morphological evaluation of canine veins following preservation in different storage media
Functional and morphological evaluation of canine veins following preservation in different storage media
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DOI：
10.1006/jsre.1996.4981
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发表时间：
1997-03-01
影响因子：
2.2
通讯作者：
Edwards, JD
中科院分区：
文献类型：
--
作者：
Cavallari, N;Abebe, W;Edwards, JD
标准保存液中保存自体静脉对内皮细胞和平滑肌细胞的损伤可能是导致移植失败的原因。比较了威斯康星州大学溶液（UWs）保存静脉与自体全血（AWE）和生理盐水（NS）保存静脉对内皮细胞和平滑肌细胞功能和形态的影响。将犬颈外静脉和股总静脉段在4 ℃的不同溶液中保存45分钟和24小时，通过在37 ℃下的等长张力研究以及通过扫描和透射电子显微镜，通过Student t检验或Mann-Whitney U检验和方差分析评估组间差异，并认为在P < 0.05时具有显著性。对去甲肾上腺素（NE）的敏感性显示，与对照静脉（6.6 +/-0.2 μ mol/L）相比，AWB（5.7 +/-0.2 μ mol/L）中45分钟的静脉储存对平滑肌功能有有害影响（P < 0.05），而NS（5.8 +/-0.2 μ mol/L）或UWs（6.5 +/-0.2 μ mol/L）中则没有。最大收缩反应和对NE的敏感性显著改变在AWE中静脉储存24小时后（P < 0.05）（0.09 +/- 0.02 g/mm（2）和5.4 +/- 0.07 μ mol/L）和NS（0.12 +/- 0.03 g/mm（2）和5.6 +/- 0.08 μ mol/L），但在UW中没有（0.36 +/- 0.06 g/mm（2）和6.4 +/- 0.07 μ mol/L）。与对照组相比，AWB和NS中保存的静脉中乙酰胆碱诱导的内皮依赖性最大舒张和敏感性显著降低（P < 0.05），但UWs中没有。同样，透射电子显微镜检查显示，在AWB和NS中储存的静脉中，血管内膜表面下有明显的中性粒细胞迁移，内皮细胞广泛分离和脱落，内皮下结构暴露。结果表明，与AWE和NS相比，UWs是一种合适的储存介质。(C)北京：科学出版社.
Injuries of endothelial and smooth muscle cells of autologous vein due to preservation in standard storage media may be responsible for graft failure, The effects of vein preservation with University of Wisconsin solution (UWs) on endothelial and smooth muscle cell function and morphology were compared to the effects of preservation with autologous whole blood (AWE) and normal saline (NS), which are frequently used in cardiovascular surgery. Canine external jugular and common femoral vein segments were preserved in the different solutions at 4 degrees C for 45 min and 24 hr, Rings (4-5 mm in length) from control and preserved veins were evaluated by isometric tension studies at 37 degrees C and by scanning and transmission electron microscopy, Differences between groups were evaluated by Student's t test or Mann-Whitney U test and by analysis of the variance, and considered to be significant at P < 0.05. Sensitivities to norepinephrine (NE) showed that a 45-min vein storage in AWB (5.7 +/- 0.2 mu mol/L) but not in NS (5.8 +/- 0.2 mu mol/L) or UWs (6.5 +/- 0.2 mu mol/L) had a deleterious effect on function of smooth muscle (P < 0.05) when compared to control veins (6.6 +/- 0.2 mu mol/L). Maximum contractile responses and sensitivities to NE were significantly altered (P < 0.05) after 24-hr vein storage in AWE (0.09 +/- 0.02 g/mm(2) and 5.4 +/- 0.07 mu mol/L) and NS (0.12 +/- 0.03 g/mm(2) and 5.6 +/- 0.08 mu mol/L) but not in UWs (0.36 +/- 0.06 g/mm(2) and 6.4 +/- 0.07 mu mol/L). With both storage times, acetylcholine-induced endothelium-dependent maximum relaxations and sensitivities were significantly reduced (P < 0.05) in veins stored in AWB and NS, but not in UWs, compared with controls. Similarly, transmission electron microscopy revealed marked neutrophil migration beneath the intimal surface of vessels and extensive separation and desquamation of endothelial cells with exposure of subendothelial structures in veins stored in AWB and NS, The results suggest that UWs is a suitable storage medium when compared to AWE and NS. (C) 1997 Academic Press.