Forces involved in bacterial adhesion to hydrophilic and hydrophobic surfaces
Forces involved in bacterial adhesion to hydrophilic and hydrophobic surfaces
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DOI：
10.1099/mic.0.2008/018622-0
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发表时间：
2008-10-01
期刊：
影响因子：
2.8
通讯作者：
Busscher, Henk J.
中科院分区：
文献类型：
--
作者：
Boks, Niels P.;Norde, Willem;Busscher, Henk J.
使用平行板流动室，评价亲水性玻璃、疏水性二甲基二氯硅烷（DDS）涂覆的玻璃和六种不同细菌菌株的防止细菌粘附（F-prev）和分离粘附细菌（F-det）的流体动力学剪切力，以检验以下三种假设。1.用于防止粘附的强流体动力学剪切力涉及用于分离粘附的生物体的强流体动力学剪切力。2.分离粘附细菌的弱流体动力学剪切力意味着通过使空气-液体界面（气泡）穿过流动室将刺激更多的细菌分离。3. DLVO（Derjaguin、朗道、Verwey、Overbeek）相互作用确定了特征流体动力学剪切力，以防止粘附并分离粘附的微生物以及通过的气液界面诱导的分离。F-prev从0.03 pN变化到0.70 pN，而F-det从0.31 pN变化到超过19.64 pN，这表明在初始接触后，发生了键的加强。通常，从DIDS涂层玻璃上分离细菌比从亲水性玻璃上分离细菌更困难，这通过气泡分离研究得到证实。基于DLVO理论（F-DLVO）计算出的吸引力对二次相互作用最小值的玻璃高于DIDS涂层玻璃。在一般情况下，所有三个假设都被拒绝，这表明，重要的是要区分平行（流体动力学剪切）和垂直（DLVO，空气-液体界面通道）的底层表面的作用力。
Using a parallel-plate flow chamber, the hydrodynamic shear forces to prevent bacterial adhesion (F-prev) and to detach adhering bacteria (F-det) were evaluated for hydrophilic glass, hydrophobic, dimethyldichlorosilane (DDS)-coated glass and six different bacterial strains, in order to test the following three hypotheses. 1. A strong hydrodynamic shear force to prevent adhesion relates to a strong hydrodynamic shear force to detach an adhering organism. 2. A weak hydrodynamic shear force to detach adhering bacteria implies that more bacteria will be stimulated to detach by passing an air-liquid interface (an air bubble) through the flow chamber. 3. DLVO (Derjaguin, Landau, Verwey, Overbeek) interactions determine the characteristic hydrodynamic shear forces to prevent adhesion and to detach adhering micro-organisms as well as the detachment induced by a passing air-liquid interface. F-prev varied from 0.03 to 0.70 pN, while F-det varied from 0.31 to over 19.64 pN, suggesting that after initial contact, strengthening of the bond occurs. Generally, it was more difficult to detach bacteria from DIDS-coated glass than from hydrophilic glass, which was confirmed by air bubble detachment studies. Calculated attractive forces based on the DLVO theory (F-DLVO) towards the secondary interaction minimum were higher on glass than on DIDS-coated glass. In general, all three hypotheses had to be rejected, showing that it is important to distinguish between forces acting parallel (hydrodynamic shear) and perpendicular (DLVO, air-liquid interface passages) to the substratum surface.