Poplar under drought: Comparison of leaf and cambial proteomic responses
Poplar under drought: Comparison of leaf and cambial proteomic responses
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DOI：
10.1016/j.jprot.2011.03.013
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发表时间：
2011-08-12
影响因子：
3.3
通讯作者：
Hausman, Jean Francois
中科院分区：
文献类型：
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作者：
Durand, Thomas C.;Sergeant, Kjell;Hausman, Jean Francois
从经济和生态的角度来看，森林生态系统具有特别重要的意义。然而，树木（多年生和木本植物）的胁迫生理机制还远未被完全了解。为此，杨树暴露在干旱中；这些植物在不同的组织中表现出常见的干旱胁迫特征。采用蛋白质组学方法对杨树叶根扦插进行研究，比较叶片和形成层两种植物组织对水分胁迫的响应。在干旱期干旱强度增加的2个时间点和复水后7天取样。我们的数据表明，两种组织对水分约束的响应时刻存在差异，形成层受影响的时间晚于叶片。在叶片中，干旱导致rubisco含量降低，光收获复合物蛋白丰度增加以及膜相关蛋白的变化。在形成层组织中，显著的蛋白质组模式变化是多种树皮储存蛋白的减少。复水后，形成层蛋白质组的变化几乎全部消失，而大量叶片蛋白质只在干旱恢复期间出现差异调节。(C) 2011 Elsevier B.V.版权所有
The forest ecosystem is of particular importance from an economic and ecological perspective. However, the stress physiology of trees, perennial and woody plants, is far from being fully understood. For that purpose, poplar plants were exposed to drought; the plants exhibited commonly reported drought stress traits in the different plant tissues. Leafy rooted cuttings of poplar were investigated through a proteomic approach in order to compare the water constraint response of two plant tissues, namely leaf and cambium. Sampling was realized during the drought period at 2 time points with increased drought intensity and 7 days after rewatering.Our data show that there is a difference in the moment of response to the water constraint between the two tissues, cambium being affected later than leaves. In leaves, drought induced a decrease in rubisco content, and an increase in the abundance of light harvesting complex proteins as well as changes in membrane-related proteins. In the cambial tissue, the salient proteome pattern change was the decrease of multiple proteins identified as bark storage proteins. After rewatering, almost all changes in cambial proteome disappeared whereas a significant number of leaf proteins appeared to be differentially regulated only during the recovery from drought. (C) 2011 Elsevier B.V. All rights reserved.