Metabolism of 3H-1 alpha,25-dihydroxyvitamin D3 in cultured human keratinocytes.
Metabolism of 3H-1 alpha,25-dihydroxyvitamin D3 in cultured human keratinocytes.
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培养的人角质形成细胞中 3H-1 α,25-二羟基维生素 D3 的代谢。
DOI：
10.1002/jcb.240590113
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Holick,MF
中科院分区：
文献类型：
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作者：
Ray,S;Ray,R;Holick,MF
在本研究中，我们研究了1α，25-二羟基-[1β-3H]维生素D3（3 H-1，25（OH）2D 3）在培养生长的人角质形成细胞（CHK）中的代谢。我们的研究结果表明，在与CHK孵育后，细胞对3 H-1，25（OH）2D 3的摄取非常迅速;并且它导致培养基中3 H-1，25（OH）2D 3的浓度降低。另一方面，3 H-骨化酸的量在培养基中缓慢增加，而细胞中3 H-骨化酸的浓度在整个孵育期间保持不可检测。当细胞与1，25（OH）2D 3（10− 8 M）预孵育时，3 H-1，25（OH）2D 3转化为3 H-柠檬酸的转化率几乎增加了两倍，表明1，25（OH）2D 3催化其自身的催化剂。© 1995 Wiley利斯公司
In the present investigation we studied the metabolism of 1α,25‐dihydroxy‐[1β‐3H] vitamin D3(3H‐1,25(OH)2D3) in culture‐grown human keratinocytes (CHK). Our results showed that the cellular uptake of3H‐1,25(OH)2D3, upon incubation with CHK, occurred very rapidly; and it paralleled a decrease in the concentration of3H‐1,25(OH)2D3in the medium. The amount of3H‐calcitroic acid, on the other hand, increased slowly in the medium, while the concentration of3H‐calcitroic acid in the cell remained undetectable during the whole period of incubation. When the cells were preincubated with 1,25(OH)2D3(10−8M), conversion of3H‐1,25(OH)2D3to3H‐calcitroic acid increased almost twofold, indicating that 1,25(OH)2D3catalyzed its own catabolism. © 1995 Wiley‐Liss, Inc.