Expression of Drosophila MAGE gene encoding a necdin homologous protein in postembryonic neurogenesis
Expression of Drosophila MAGE gene encoding a necdin homologous protein in postembryonic neurogenesis
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DOI：
10.1016/j.modgep.2006.09.008
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发表时间：
2007-01-01
影响因子：
1.2
通讯作者：
Yoshikawa, Kazuaki
中科院分区：
文献类型：
--
作者：
Nishimura, Isao;Shimizu, Shoko;Yoshikawa, Kazuaki
法师（melanoma antigen）家族的特征在于称为法师同源结构域的大的保守结构域。最初鉴定的编码肿瘤排斥抗原的法师基因仅在癌症和男性生殖细胞中表达。包含法师同源结构域的Necdin在有丝分裂后细胞如神经元和骨骼肌细胞中高度表达。人类necdin基因NDN仅通过基因组印记从父系等位基因转录，其缺陷与神经发育障碍Prader Willi综合征的发病机制有关。尽管在人类中已经鉴定了超过30个法师基因，但果蝇（Drosophila melanogaster）仅具有单个法师基因，其编码与necdin同源法师蛋白相似的蛋白。本研究分析了法师mRNA及其编码蛋白在果蝇发育过程中的时空表达模式。全胚胎原位杂交分析表明，法师mRNA高表达的合胞胚盘阶段和腹侧和prophalic神经原区域的外胚层原肠胚形成期间。相反，法师表达几乎是不可检测的有丝分裂后的中枢神经系统的神经元在胚胎晚期。在胚后神经发生过程中，法师在幼虫和蛹期的神经干细胞（成神经细胞）及其后代（神经节母细胞和有丝分裂后神经元）中高度表达。法师也表达于有丝分裂后的神经元，包括成年期的蘑菇体神经元和视网膜光感受器。这些结果表明，法师表达持续整个胚后神经发生在果蝇。(c)2006 Elsevier B.V.保留所有权利。
The MAGE (melanoma antigen) family is characterized by a large conserved domain termed MAGE homology domain. Originally identified MAGE genes encoding tumor rejection antigens are expressed only in cancers and male germ cells. Necdin, which contains the MAGE homology domain, is highly expressed in postmitotic cells such as neurons and skeletal muscle cells. The human necdin gene NDN is transcribed only from the paternal allele through genomic imprinting, and its deficiency is implicated in the pathogenesis of the neurodevelopmental disorder Prader Willi syndrome. Although over 30 MAGE genes have been identified in humans, fruit fly (Drosophila melanogaster) has only a single MAGE gene that encodes a protein similar to necdin homologous MAGE proteins. In this study, we analyzed the spatiotemporal expression patterns of MAGE mRNA and the encoded protein during fly development. Whole-mount embryo in situ hybridization analysis revealed that MAGE mRNA was highly expressed at the syncytial blastoderm stage and in the ventral and procephalic neurogenic regions of the ectoderm during gastrulation. In contrast, MAGE expression was nearly undetectable in postmitotic neurons of the central nervous system at late embryonic stages. During postembryonic neurogenesis, MAGE was highly expressed in neural stem cells (neuroblasts) and their progeny (ganglion mother cells and postmitotic neurons) at larval and pupal stages. MAGE was also expressed in postmitotic neurons including mushroom body neurons and retinal photoreceptors in adulthood. These results indicate that MAGE expression lasts throughout the postembryonic neurogenesis in Drosophila. (c) 2006 Elsevier B.V. All rights reserved.