COMPARISON BETWEEN DIRECT METHODS FOR DETERMINATION OF MICROBIAL CELL-VOLUME - ELECTRON-MICROSCOPY AND ELECTRONIC PARTICLE SIZING
COMPARISON BETWEEN DIRECT METHODS FOR DETERMINATION OF MICROBIAL CELL-VOLUME - ELECTRON-MICROSCOPY AND ELECTRONIC PARTICLE SIZING
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DOI：
10.1128/aem.45.5.1651-1658.1983
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发表时间：
1983-01-01
影响因子：
4.4
通讯作者：
GUERRERO, R
中科院分区：
文献类型：
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作者：
MONTESINOS, E;ESTEVE, I;GUERRERO, R
通过扫描和透射电磁以及电子粒度测定（EPS）测定不同光养细菌和异养细菌和酵母的尺寸频率分布。这 3 种技术之间存在统计学上的显着差异。经 EM 处理的细胞显示出比经 EPS 处理的细胞更低的平均细胞体积，反映了 1.1-6.2 倍（平均 2.3 倍）的缩小。用于扫描 EM 的细胞处理比用于透射 EM 的处理造成更高的收缩。收缩既不依赖于微生物的大小也不依赖于细胞壁类型。当进行扫描电镜处理时，光养细菌强烈收缩，而异养微生物受到的影响较小。光养细菌的收缩百分比和特定色素含量之间存在直接关系。这可能是脱水过程中有机溶剂萃取颜料的结果，在临界点干燥之前，需要通过扫描电镜检查样品。
Size frequency distributions of different phototrophic bacteria and heterotrophic bacteria and yeasts were determined by scanning and transmission EM and electronic particle sizing (EPS). Statistically significant differences existed among these 3 techniques. Cells processed for EM showed lower mean cellular volumes than those processed for EPS, reflecting a shrinking by factors ranging from 1.1-6.2 (mean, 2.3.). Processing of cells for scanning EM caused higher shrinkage than processing for transmission EM. Shrinkage was dependent neither on the size nor on the cell wall type of the microorganism. When processed for scanning EM, phototrophic bacteria were strongly shrunken, whereas heterotrophic microorganisms were less affected. A direct relationship existed among phototrophic bacteria between percentage of shrinkage and specific pigment content. This was probably a consequence of the pigment extraction by organic solvents during the dehydration process, previous to the critical point drying, necessary to examine the specimens by scanning EM.