Bone formation in CaP-coated and noncoated titanium fiber mesh
Bone formation in CaP-coated and noncoated titanium fiber mesh
复制标题
DOI：
10.1002/jbm.a.10288
复制
发表时间：
2003-03-01
影响因子：
4.9
通讯作者：
Jansen, JA
中科院分区：
文献类型：
--
作者：
Vehof, JWM;van den Dolder, J;Jansen, JA
在同系大鼠异位试验模型中，比较了磷酸钙（CaP）涂层和无涂层多孔钛（Ti）纤维网的成骨活性，该纤维网在长时间原位培养后装载有培养的同系成骨细胞。大鼠骨髓（RBM）细胞加载到钙磷涂层和非涂层钛支架使用液滴或悬浮液加载方法。加载后，体外培养RBM细胞8天。此后，将植入物皮下放置在39只同系大鼠中。在术后2、4和8周对大鼠实施安乐死，并取出植入物。此外，在8周组中，在2、4和6周时注射荧光染料骨标记物。组织学分析表明，只有CaP涂层补片支持骨形成。新形成的骨的量在单个和多个球体之间变化，以填充补片孔隙的显著部分。在新形成的骨中，可以清楚地观察到嵌入矿化基质中的骨细胞。另一方面，在无涂层钛植入物中，观察到大量含钙材料沉积。这种存款缺乏骨样组织结构。进一步的分析表明，细胞加载方法不影响最终的骨形成量。在CaP涂层种植体中，荧光染料标记物的累积顺序表明骨形成始于网状纤维。总之，我们的研究结果证明，薄CaP涂层，钛网，和RBM细胞的组合确实可以产生异位骨形成后，长期在体外培养。未观察到加载方法对最终骨量的影响。(C)2003 Wiley Periodicals，Inc.
The osteogenic activity of calcium phosphate (CaP)-coated and noncoated porous titanium (Ti) fiber mesh loaded with cultured syngeneic osteogenic cells after prolonged in situ culturing was compared in a syngeneic rat ectopic assay model. Rat bone marrow (RBM) cells were loaded onto the CaP-coated and noncoated Ti scaffolds using either a droplet or a suspension loading method. After loading, the RBM cells were cultured for 8 days in vitro. Thereafter, implants were subcutaneously placed in 39 syngeneic rats. The rats were euthanized and the implants retrieved at 2, 4, and 8 weeks postoperatively. Further, in the 8 week group fluorochrome bone markers were injected at 2, 4, and 6 weeks. Histological analysis demonstrated that only the CaP-coated meshes supported bone formation. The amount of newly formed bone varied between single and multiple spheres to filling a significant part of the mesh porosity. In the newly formed bone, osteocytes embedded in a mineralized matrix could be observed clearly. On the other hand, in the noncoated titanium implants, abundant deposition of calcium-containing material was seen. This deposit lacked a bonelike tissue organization. Further analysis revealed that the cell-loading method did not influence the final amount of bone formation. In CaP-coated implants the accumulation sequence of the fluorochrome markers showed that bone formation started on the mesh fibers. In conclusion, our results prove that the combination of a thin CaP coating, Ti-mesh, and RBM cells can indeed generate ectopic bone formation after prolonged in vitro culturing. No effect of the loading method was observed on the final amount of bone. (C) 2003 Wiley Periodicals, Inc.