Interleukin-8 levels and activity in delayed-healing human thermal wounds
Interleukin-8 levels and activity in delayed-healing human thermal wounds
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DOI：
10.1046/j.1524-475x.2000.00216.x
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发表时间：
2000-05-01
影响因子：
2.9
通讯作者：
Garner, WL
中科院分区：
文献类型：
--
作者：
Iocono, JA;Colleran, KR;Garner, WL
有许多原因导致伤口愈合缓慢或延迟。由于缓慢愈合的伤口通常会发炎，我们定量了缓慢愈合的人类烧伤伤口产生的炎症趋化因子白细胞介素-8，并将其与愈合伤口和正常完整皮肤产生的白细胞介素-8进行了比较。与愈合伤口（7.7 ng/ml）或正常皮肤（5.7 ng/ml）相比，未愈合伤口（19.7 ng/ml）中的白细胞介素-8水平显著升高。将这些范围内的白细胞介素-8添加到成人角质形成细胞和成纤维细胞中。白细胞介素-8显着降低角质形成细胞的复制，但对成纤维细胞的复制没有影响。成纤维细胞填充的胶原晶格收缩的速率或最终程度在10和30 ng/ml之间的白细胞介素-8浓度下被抑制，但在低于10 ng/ml和高于100 ng/ml的浓度下不改变。同时应用10 μ g/ml的吲哚美辛可逆转这种白细胞介素-8诱导的抑制。白细胞介素-8抑制肌球蛋白ATP酶活性，显然是通过减少非肌肉肌球蛋白轻链的磷酸化。我们的结论是，白细胞介素-8水平升高可能会发现在延迟愈合，这些升高的白细胞介素-8水平可能直接导致延迟伤口愈合。
There are numerous causes for slow or delayed wound healing. Because slowly healing wounds are often inflamed, we quantitated the inflammatory chemokine, interleukin-8, produced by slowly healing human burn wounds and compared this to interleukin-8 from healed wounds and normal intact skin. Interleukin-8 levels were increased significantly in unhealed wounds (19.7 ng/ml) compared to healed wounds (7.7 ng/ml) or normal skin (5.7 ng/ml). Interleukin-8 in these ranges was added to adult human keratinocytes and fibroblasts. Interleukin-8 significantly decreased keratinocyte replication but had no effect on fibroblast replication. The rate or final degree of fibroblast populated collagen lattice contraction was inhibited at interleukin-8 concentrations between 10 and 30 ng/ml, but not altered at concentrations below 10 ng/ml and above 100 ng/ml. The concurrent application of indomethacin at 10 mu g/ml reversed this interleukin-8 induced inhibition. Interleukin-8 inhibited myosin ATPase activity, apparently by reducing the phosphorylation of nonmuscle myosin light chain. We conclude that elevated levels of interleukin-8 may be found during delayed healing, and these elevated interleukin-8 levels may directly contribute to retarded wound closure.