Characteristics of Plasmodium falciparum parasites that survive the lengthy dry season in eastern Sudan where malaria transmission is markedly seasonal
Characteristics of Plasmodium falciparum parasites that survive the lengthy dry season in eastern Sudan where malaria transmission is markedly seasonal
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DOI：
10.4269/ajtmh.1998.59.582
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发表时间：
1998-10-01
影响因子：
3.3
通讯作者：
Walliker, D
中科院分区：
文献类型：
--
作者：
Babiker, HA;Abdel-Muhsin, ABA;Walliker, D
我们检查了83名居民的Asar村在苏丹东部，疟疾传播持续约2-3个月，每年，在长期的旱季恶性疟原虫的存在。所有患者在首次诊断后接受标准剂量的氯喹治疗，然后在前两个月每两周进行一次显微镜检查和聚合酶链反应（PCR）检查，随后在整个旱季直至下一个传播季节的15个月内每月进行一次检查。PCR引物使用裂殖子表面蛋白-1（MSP-1）、MSP-2和富含谷氨酸蛋白基因的扩增多态性区域。结果表明，在整个旱季，部分患者的亚显性和无症状寄生虫血症持续数月，通常是遗传复杂感染。不同的基因型可以在一次感染中共存，并且在此期间每种基因型的比例可能会发生剧烈波动。然而，在一些个体中，单一基因型似乎持续数月。慢性感染患者临床症状的重现通常与新等位基因的出现有关，表明新基因型寄生虫的再感染。
We have examined 83 inhabitants of Asar village in eastern Sudan, where malaria transmission lasts approximately 2-3 months each year, for the presence of Plasmodium falciparum during the prolonged dry season. All patients were treated with a standard dose of chloroquine following the first diagnosis, then examined by microscopy and the polymerase chain reaction (PCR) every two weeks for the first two months and subsequently once each month for the next 15 months throughout the dry season until the following transmission season. The PCR primers used amplified polymorphic regions of the merozoite surface protein-1 (MSP-1), MSP-2, and glutamate-rich protein genes. Results show that subpatent and asymptomatic parasitemias persisted in some patients for several months throughout the dry season, often as genetically complex infections. Different genotypes could coexist together in a single infection and the proportions of each could fluctuate dramatically during this period. However, in some individuals, single genotypes appeared to persist for several months. Reappearance of clinical symptoms among patients with chronic infections was often associated with appearance of new alleles, indicating reinfections with parasites of novel genotypes.