INTERACTION OF BETA-LACTOGLOBULIN WITH RETINOL AND FATTY-ACIDS AND ITS ROLE AS A POSSIBLE BIOLOGICAL FUNCTION FOR THIS PROTEIN - A REVIEW
INTERACTION OF BETA-LACTOGLOBULIN WITH RETINOL AND FATTY-ACIDS AND ITS ROLE AS A POSSIBLE BIOLOGICAL FUNCTION FOR THIS PROTEIN - A REVIEW
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DOI：
10.3168/jds.s0022-0302(95)76713-3
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发表时间：
1995-05-01
影响因子：
3.5
通讯作者：
CALVO, M
中科院分区：
文献类型：
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作者：
PEREZ, MD;CALVO, M
β-乳球蛋白是反刍动物和一些非反刍动物（例如猪和马）奶中的主要乳清蛋白。尽管 60 年前首次分离出 β-乳球蛋白，但尚未明确确定 β-乳球蛋白具有任何功能。最近的 X 射线晶体学研究进一步了解了 β-乳球蛋白的结构，该蛋白与视黄醇结合蛋白和脂萼蛋白的结构同源；这些蛋白质的功能似乎是参与小疏水物质的运输。通过类推，这种蛋白质被认为具有脂肪酸和视黄醇转运蛋白的作用。本综述根据过去几年积累的大量信息重新评估了 β-乳球蛋白的功能。特别是，本综述重点研究视黄醇和脂肪酸与 β-乳球蛋白的结合，包括结合常数和结合位点的数量、结合位点的位置以及化学修饰对蛋白质与两种配体相互作用的影响。这项研究还描述了 β-乳球蛋白对几种生物过程的影响的研究，这些生物过程可能与该蛋白质的可能生物学作用相关。
beta-Lactoglobulin is the major whey protein in the milk of ruminants and some nonruminants, such as pigs and horses. Although beta-lactoglobulin was first isolated 60 yr ago, no function has been definitely ascribed to beta-lactoglobulin. Recent x-ray crystallographic studies have advanced knowledge of the structure of beta-lactoglobulin, which is homologous with that of retinol-binding protein and lipocalycins; the function of these proteins seems to be participation in the transport of small hydrophobic substances. By analogy, this protein has been suggested as having a role as a transporter of fatty acids and retinol. This review reassesses the function of beta-lactoglobulin in light of the large amount of information that has accrued in the last few years. In particular, this review concentrates upon studies of the binding of retinol and fatty acids to beta-lactoglobulin, including the binding constants and number of binding sites, the location of the binding sites, and the influence of chemical modifications in the interaction of the protein with both ligands. This study also describes studies of the influence of beta-lactoglobulin on several biological processes that may be relevant to the possible biological role of this protein.