Transcription factor Gbx2 acts cell-nonautonomously to regulate the formation of lineage-restriction boundaries of the thalamus
Transcription factor Gbx2 acts cell-nonautonomously to regulate the formation of lineage-restriction boundaries of the thalamus
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DOI：
10.1242/dev.030510
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发表时间：
2009-04-15
期刊：
影响因子：
4.6
通讯作者：
Li, James Y. H.
中科院分区：
文献类型：
--
作者：
Chen, Li;Guo, Qiuxia;Li, James Y. H.
对于丘脑的发育，尤其是其分化成不同的核，人们知之甚少。我们在这里证明了小鼠间脑中表达Gbx2的细胞对整个丘脑核复合体的贡献。然而，不同丘脑核团的神经元前体在时间上表现出不同的Gbx2表达模式。表达Gbx2的细胞及其后代形成清晰的谱系限制边界，将丘脑与顶盖前、上丘脑和前丘脑区分开来，揭示了小鼠间脑内的多个隔室边界。如果没有Gbx2，来自丘脑的细胞就会异常地为上皮层和顶盖前胚胎做出贡献。这种异常不是由于Gbx2突变体的图案或细胞命运说明中的明显缺陷造成的。嵌合和遗传镶嵌分析表明，Gbx2在控制有丝分裂后丘脑神经元与不表达Gbx2的邻近脑结构的分离方面发挥着细胞非自治作用。我们认为，在发育中的丘脑中，Gbx2的动态和差异表达可能参与了丘脑神经元的特异性分离，导致丘脑划分为不同的核团。
Relatively little is known about the development of the thalamus, especially its differentiation into distinct nuclei. We demonstrate here that Gbx2-expressing cells in mouse diencephalon contribute to the entire thalamic nuclear complex. However, the neuronal precursors for different thalamic nuclei display temporally distinct Gbx2 expression patterns. Gbx2-expressing cells and their descendents form sharp lineage-restriction boundaries delineating the thalamus from the pretectum, epithalamus and prethalamus, revealing multiple compartmental boundaries within the mouse diencephalon. Without Gbx2, cells originating from the thalamus abnormally contribute to the epithalamus and pretectum. This abnormality does not result from an overt defect in patterning or cell-fate specification in Gbx2 mutants. Chimeric and genetic mosaic analysis demonstrate that Gbx2 plays a cell-nonautonomous role in controlling segregation of postmitotic thalamic neurons from the neighboring brain structures that do not express Gbx2. We propose that, within the developing thalamus, the dynamic and differential expression of Gbx2 may be involved in the specific segregation of thalamic neurons, leading to partition of the thalamus into different nuclei.