Can biomarkers differentiate pain and no pain subgroups of nonverbal children with cerebral palsy? A preliminary investigation based on noninvasive saliva sampling.
Can biomarkers differentiate pain and no pain subgroups of nonverbal children with cerebral palsy? A preliminary investigation based on noninvasive saliva sampling.
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生物标志物可以区分脑瘫非语言儿童的疼痛和无痛亚组吗？
DOI：
10.1111/pme.12545
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Wilcox,GeorgeL
中科院分区：
文献类型：
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作者：
Symons,FrankJ;ElGhazi,Issam;Reilly,BrianG;Barney,ChantelC;Hanson,Leah;Panoskaltsis-Mortari,Angela;Armitage,IanM;Wilcox,GeorgeL
目的评估和治疗患有发育障碍的非语言儿童的疼痛是一项临床挑战。目前的评估方法依赖于代理报告完成的临床印象和行为评级量表。鉴于唾液代谢组与健康的相关性日益增强，我们利用质子核磁共振 (NMR) 波谱和神经肽/细胞因子/激素检测进行了一项以转化为导向的可行性研究，以比较一组与伤害感受相关的唾液生物标志物。设计组内观察设计。设置三级儿科康复医院。受试者 10 名伴有或不伴有疼痛的脑瘫非语言儿科患者。方法非刺激（被动收集）唾液使用口腔拭子收集，然后用高氯酸提取，并在 Bruker Avance 700 MHz NMR 波谱仪上进行分析。我们还测量了几种细胞因子、趋化因子、激素和神经肽的唾液水平。结果偏最小二乘判别分析显示，那些有/没有疼痛的儿童在许多不同的生物标志物上是分开的。大多数唾液代谢物、神经肽、细胞因子和激素水平在有疼痛的儿童中比没有疼痛的儿童更高。结论样本收集和分析的简便性和非侵入性方式支持了这种新型生物标志物监测方法在临床实践中定期预测使用的可能性。
ObjectiveAssessing and treating pain in nonverbal children with developmental disabilities are a clinical challenge. Current assessment approaches rely on clinical impression and behavioral rating scales completed by proxy report. Given the growing health relevance of the salivary metabolome, we undertook a translational-oriented feasibility study using proton nuclear magnetic resonance (NMR) spectroscopy and neuropeptide/cytokine/hormone detection to compare a set of salivary biomarkers relevant to nociception.DesignWithin-group observational design.SettingTertiary pediatric rehabilitation hospital.SubjectsTen nonverbal pediatric patients with cerebral palsy with and without pain.MethodsUnstimulated (passively collected) saliva was collected using oral swabs followed by perchloric acid extraction and analyzed on a Bruker Avance 700 MHz NMR spectrometer. We also measured salivary levels of several cytokines, chemokines, hormones, and neuropeptides.ResultsPartial least squares discriminant analysis showed separation of those children with/without pain for a number of different biomarkers. The majority of the salivary metabolite, neuropeptide, cytokine, and hormone levels were higher in children with pain vs no pain.ConclusionsThe ease of collection and noninvasive manner in which the samples were collected and analyzed support the possibility of the regular predictive use of this novel biomarker-monitoring method in clinical practice.