Alpha6beta1 integrin expressed by sperm is determinant in mouse fertilization.
Alpha6beta1 integrin expressed by sperm is determinant in mouse fertilization.
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DOI：
10.1186/1471-213x-7-102
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发表时间：
2007-09-12
影响因子：
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通讯作者：
Ziyyat A
中科院分区：
文献类型：
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作者：
Barraud-Lange V;Naud-Barriant N;Saffar L;Gattegno L;Ducot B;Drillet AS;Bomsel M;Wolf JP;Ziyyat A
根据抑制试验，α6β1整合素被认为是一种精子受体，但进一步使用基因缺失技术的实验表明，卵母细胞α6和β1整合素亚单位都不是小鼠受精所必需的。Western印迹分析和免疫荧光分析表明，小鼠精子表达α6β1整合素。对于卵母细胞，GoH3抗α6抗体与精子结合可引起精子受精能力的特异性抑制。在融合试验中比较去透明带和去透明带的卵子，我们发现酸处理去除透明带绕过了受精卵母细胞α6beta1整合素在黏附/融合过程中的作用。这些发现表明，α6beta1整合素在两个配子中都有表达，并在它们的膜相互作用中发挥功能。这些结果和以前关于野生型精子受精α6或β1整合素亚基缺失的卵母细胞的报道表明，在两种配子膜之一上存在α6β1整合素可以挽救受精过程。这一假设进一步得到了我们最近报道的配子之间在融合前发生的膜碎片交换的支持。
Based on inhibition tests, the alpha6beta1 integrin was suggested to be a sperm receptor, but further experiments using gene deletion techniques have shown that neither oocyte alpha6, nor beta1 integrin subunits were essential for mouse fertilization. Using Western blot analysis and immunofluorescence, we showed that the mouse sperm expresses the alpha6beta1 integrin. As for oocyte, binding of GoH3 anti-alpha6 antibody to sperm induces a specific inhibition of sperm fertilizing ability. Comparing zona-intact and zona-free eggs in fusion tests, we showed that the removal of the zona pellucida by acid treatment bypasses fertilizing oocyte alpha6beta1 integrin's function in the adhesion/fusion process. These findings show that alpha6beta1 integrin is expressed by both gametes and is functional in their membranes interaction. These results and previous reports, about fertilization of alpha6 or beta1 integrin subunits deleted oocytes by wild type sperm, suggest that the presence of alpha6beta1 integrin on one of the two gamete membranes can rescue the fertilization process. This hypothesis is further supported by the exchange of membrane fragments occurring between gametes prior to fusion that we recently reported.