Ligand-directed tosyl chemistry for protein labeling in vivo
Ligand-directed tosyl chemistry for protein labeling in vivo
复制标题
DOI：
10.1038/nchembio.157
复制
发表时间：
2009-05-01
影响因子：
14.8
通讯作者：
Hamachi, Itaru
中科院分区：
文献类型：
--
作者：
Tsukiji, Shinya;Miyagawa, Masayoshi;Hamachi, Itaru
在这里，我们描述了一种利用配体定向tosyl （LDT）化学将合成分子选择性地附着到体内特定的“内源性”蛋白质上的方法。该方法不仅可用于对活细胞、组织和小鼠中的蛋白质进行化学标记，还可用于直接在细胞内构建生物传感器，而无需进行基因工程。这些数据确立了LDT化学作为研究和操纵生物系统的新工具。
Here we describe a method for the site-selective attachment of synthetic molecules into specific 'endogenous' proteins in vivo using ligand-directed tosyl (LDT) chemistry. This approach was applied not only for chemically labeling proteins in living cells, tissues and mice but also for constructing a biosensor directly inside cells without genetic engineering. These data establish LDT chemistry as a new tool for the study and manipulation of biological systems.