Plasma soluble α-klotho protein levels in premature and term neonates: correlations with growth and metabolic parameters.
Plasma soluble α-klotho protein levels in premature and term neonates: correlations with growth and metabolic parameters.
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DOI：
10.1530/eje-12-0476
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发表时间：
2012-09
影响因子：
5.8
通讯作者：
Chrousos G
中科院分区：
文献类型：
--
作者：
Siahanidou T;Garatzioti M;Lazaropoulou C;Kourlaba G;Papassotiriou I;Kino T;Imura A;Nabeshima Y;Chrousos G
α-Klotho （α-KL）是一种具有抗衰老特性的蛋白质，可调节磷酸盐、钙和骨代谢，诱导对氧化应激的抵抗，并可能参与胰岛素信号传导。α-KL在新生儿中所起的作用尚不清楚，已知新生儿易发生代谢紊乱和氧化应激。本研究的目的是评估早产儿和足月新生儿循环可溶性α-KL浓度，并揭示其与生长、代谢和氧化应激指标的可能相关性。前瞻性研究。采用特异性ELISA法测定50例健康新生儿（25例早产儿，平均胎龄33.7（1.1）周，25例足月新生儿）出生第14天和28天血浆可溶性α-KL水平。研究α-KL与人体测量、代谢参数和氧化应激指标的关系。在第14天，足月儿α-KL水平显著高于早产儿(1099 (480)pg/ml vs 884 (239) pg/ml)；P<0.05)和28 (1277 (444)pg/ml vs 983 (264) pg/ml；P < 0.01)。在早产儿和足月儿中，α-KL水平在14 ~ 28天显著升高（P<0.001）。循环α-KL浓度与GA （β=0.32， P=0.001）、体重（β=0.34， P=0.001）、体长（β=0.33， P=0.001）、1,25-二羟基维生素D水平（β=0.24， P<0.05）、丙二醛水平（β=0.20， P<0.05）相关，与葡萄糖、胰岛素或胰岛素抵抗稳态模型评价指标无相关性。可溶性α-KL水平随着GA和出生年龄的增加而升高，可能对维生素D代谢和氧化应激有影响。α-KL是否在婴幼儿生长发育的调控中发挥作用还有待进一步研究。
α-Klotho (α-KL), a protein with antiaging properties, regulates phosphate, calcium, and bone metabolism, induces resistance to oxidative stress, and may participate in insulin signaling. The role of α-KL in neonates, known to be prone to metabolic disturbances and oxidative stress, is not known. The aim of this study was to evaluate circulating soluble α-KL concentrations in preterm and full-term neonates and unravel possible correlations with growth, metabolism, and indices of oxidative stress. Prospective study. Plasma-soluble α-KL levels were determined by specific ELISA in 50 healthy neonates (25 preterm, mean (S.D.) gestational age (GA) 33.7 (1.1) weeks, and 25 full-term infants) at days 14 and 28 of life. Associations of α-KL with anthropometric, metabolic parameters, and indices of oxidative stress were examined. α-KL levels were significantly higher in full-term than in preterm infants at both days 14 (1099 (480) pg/ml vs 884 (239) pg/ml respectively; P<0.05) and 28 (1277 (444) pg/ml vs 983 (264) pg/ml respectively; P<0.01). In both preterm and full-term infants, α-KL levels increased significantly from day 14 to 28 of life (P<0.001). Circulating α-KL concentrations correlated with GA (β=0.32, P=0.001), body weight (β=0.34, P=0.001), body length (β=0.33, P=0.001), 1,25-dihydroxy-vitamin D level (β=0.24, P<0.05), and malondialdehyde level (β=0.20, P<0.05) but not with glucose, insulin, or homeostasis model assessment index of insulin resistance values. Soluble α-KL levels rise as GA and postnatal age advance in neonates and may have an impact on vitamin D metabolism and oxidative stress. Whether α-KL may have a role in the regulation of infants’ growth should be further studied.