Site-specific cleavage of basement membrane collagen IV during Drosophila metamorphosis.
Site-specific cleavage of basement membrane collagen IV during Drosophila metamorphosis.
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发表时间：
1993-03
期刊：
影响因子：
4.6
通讯作者：
L. Fessler;Maureen L. Condic;Robert E. Nelson;J. Fessler;J. Fristrom
中科院分区：
文献类型：
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作者：
L. Fessler;Maureen L. Condic;Robert E. Nelson;J. Fessler;J. Fristrom
在肿瘤细胞的变形、迁移和转移扩散过程中，基底膜的破坏是控制细胞重排的重要步骤。我们的两个实验室之一发现了一种独特的胶原肽，只有在果蝇变态时才会出现。另一个实验室以前曾报道，在20-羟基蜕皮酮诱导的果蝇成像盘外翻过程中，一种名为gp125的糖蛋白出现(Birr等人，1990年)。我们证明了这两个肽是相同的，它们是由基底膜IV型胶原形成的。在Calde/IKMPAK序列中755/756残基之间的同源三聚体胶原螺旋缺失时发生切割。该肽是从胶原α1(IV)链的氨基酸序列衍生的羧基片段100,647M(R)。相应的氨基片段也从二硫键连接的聚集体中被回收。这种特殊的切割支持了在变态过程中基底膜高度靶向、可控的破裂的概念。此外，这些切割发生在果蝇基底膜预测的IV型胶原超分子网络的关键位置。
Breakdown of basement membranes is an important step in the controlled rearrangement of cells during metamorphosis, cell migration, and metastatic spread of tumor cells. One of our two laboratories found a unique collagenous peptide that only appears during metamorphosis of Drosophila melanogaster. The other laboratory previously reported that during 20-hydroxyecdysone-induced eversion of Drosophila imaginal discs a glycoprotein named gp125 arises (Birr et al., 1990). We show that these two peptides are identical and that they are formed from basement membrane collagen IV. Cleavage occurs at an imperfection of this homotrimeric collagen helix between residues 755/756 in the sequence CALDE/IKMPAK. The peptide is the carboxyl fragment, 100,647 M(r), as derived from the amino acid sequence of the collagen alpha 1(IV) chain. The corresponding amino fragment was also recovered from a disulfide-linked aggregate. This specific cleavage supports the concept of highly targeted, controlled breakdown of basement membranes during metamorphosis. Furthermore, these cuts occur at strategic sites of the predicted supramolecular network of collagen IV molecules of Drosophila basement membranes.