Interleukin-12 as an adjuvant promotes immunoglobulin G and type 1 cytokine recall responses to major surface protein 2 of the ehrlichial pathogen Anaplasma marginale.
Interleukin-12 as an adjuvant promotes immunoglobulin G and type 1 cytokine recall responses to major surface protein 2 of the ehrlichial pathogen Anaplasma marginale.
复制标题
Interleukin-12 作为佐剂可促进免疫球蛋白 G 和 1 型细胞因子对埃利希体病原体边缘无形体主要表面蛋白 2 的召回反应。
DOI：
10.1128/iai.68.1.270-280.2000
复制
发表时间：
2000
影响因子：
3.1
通讯作者：
Brown,WC
中科院分区：
文献类型：
--
作者：
Tuo,W;Palmer,GH;McGuire,TC;Zhu,D;Brown,WC
Anaplasma marginaleis a tick-transmitted pathogen of cattle closely related to the human ehrlichiae,Ehrlichia chaffeensisand the agent of human granulocytic ehrlichiosis (HGE). These pathogens have in common a structurally conserved outer membrane protein (OMP) designated the major surface protein 2 (MSP-2) inA. marginaleand HGE and OMP-1 inE. chaffeensis. Protective immunity against ehrlichial pathogens is believed to require induction of gamma interferon (IFN-γ) and opsonizing immunoglobulin (Ig) subclasses directed against OMP epitopes that, in concert, activate macrophages for phagocytosis and killing. Because interleukin-12 (IL-12) acts as an adjuvant for protein immunization to induce IFN-γ and protective immunity against intracellular pathogens, we hypothesized that as an adjuvant with MSP-2, IL-12 would augment type 1 recall responses toA. marginale. IL-12 was coadsorbed with MSP-2 to alum and shown to significantly enhance IFN-γ production by lymph node cells (LNC) and LNC-derived CD4+T-cell lines from immunized calves following recall stimulation withA. marginale. LNC proliferation and IL-2 production were also enhanced in IL-12-treated calves. Elevated recall proliferative responses by peripheral blood mononuclear cells were still evident 9 months after immunization. Serum IgG levels were consistently increased in IL-12 immunized calves, predominantly due to higher IgG1 responses. The results support the use of IL-12 coadsorbed with OMP of ehrlichial pathogens in alum to amplify both antibody and type-1 cytokine responses important for protective immunity.
