Differential Distribution of Renal Nerves in the Sympathetic Ganglia of the Rat
Differential Distribution of Renal Nerves in the Sympathetic Ganglia of the Rat
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大鼠交感神经节肾神经的差异分布
DOI：
10.1002/ar.23680
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Yagi Hideshi
中科院分区：
文献类型：
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作者：
Maeda Seishi;Fujihira Mayumi;Minato Yusuke;Kuwahara-Otani Sachi;Tanaka Koichi;Hayakawa Tetsu;Yagi Hideshi
肾神经丛包括传出纤维和传入纤维。它控制尿液的产生和体液的体内平衡。到肾脏的传出纤维包括来自其主神经节、椎前肾上腺神经节（SrG）和椎旁交感神经链神经节（ChG）的交感神经纤维。在本研究中，我们研究了拓扑神经支配从这些神经节的肾实质部分的左肾的大鼠。将Fluoro-Gold注射到左肾的吻极或尾极。在头极注射的大鼠的SrG中约50%的细胞被标记，而在尾极注射的大鼠的ChG T13中60%的细胞被标记。此外，我们使用两种荧光结合的霍乱毒素（f-CTbs）注射到左肾的吻极和尾极进行神经的双探头逆行追踪。用每种f-CTb标记的细胞在左侧SrG和下部ChG中分布不同;在这些神经节中未发现双标记细胞。用转染到左侧SrG中的pCAGGS-tdTomato载体进行的顺行追踪显示，tdTomato标记的神经静脉曲张延伸到皮质小动脉和肾小管。免疫组织化学染色显示，它们呈酪氨酸羟化酶和突触素阳性，提示它们具有交感神经末梢。我们的研究结果表明，肾传出神经在SrG可能控制的吻部的肾脏和神经支配的多个效应器在皮质。Anat Rec，300：2263-2272，2017。© 2017 Wiley Periodicals，Inc.
The renal nerve plexus comprises efferent and afferent fibers. It controls urine production and bodily fluid homeostasis. Efferent fibers to the kidney include sympathetic nerve fibers from their main ganglia, the prevertebral suprarenal ganglia (SrG), and the paravertebral sympathetic chain ganglia (ChG). In the present study, we examined topological innervation from these ganglia to the renal parenchymal segments of the left kidney of the rat. Fluoro‐Gold was injected into the rostral or caudal poles of the left kidney. Approximately 50% of the cells in the SrG of rats injected in the rostral pole were labeled, while 60% of the cells in the ChG T13 of rats injected in the caudal pole were labeled. In addition, we performed dual‐probe retrograde tracing of the nerves using two kinds of fluorescent‐conjugated cholera toxins (f‐CTbs) injected into the rostral and caudal poles of the left kidney. The cells labeled with each f‐CTb were distributed differently in the left SrG and the lower ChGs; no dual‐labeled cells were found in these ganglia. Anterograde tracing with pCAGGS‐tdTomato vector transfected into the left SrG showed that tdTomato‐labeled nerve varicosities extended to the cortical arterioles and urinary tubules. Immunohistochemistry revealed that they were positive to tyrosine hydroxylase and synaptophysin, suggesting that they possessed sympathetic nerve endings. Our results show that renal efferent nerves in the SrG may control the rostral part of the kidney and innervate the multiple effectors in the cortex. Anat Rec, 300:2263–2272, 2017. © 2017 Wiley Periodicals, Inc.