DIFFERENTIAL LOCALIZATION OF N-ACETYLATED INDOLEALKYLAMINES IN CNS AND HARDERIAN GLAND USING IMMUNOHISTOLOGY
DIFFERENTIAL LOCALIZATION OF N-ACETYLATED INDOLEALKYLAMINES IN CNS AND HARDERIAN GLAND USING IMMUNOHISTOLOGY
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DOI：
10.1016/0006-8993(76)90309-7
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发表时间：
1976-01-01
期刊：
影响因子：
2.9
通讯作者：
GROTA, LG
中科院分区：
文献类型：
--
作者：
BUBENIK, GA;BROWN, GM;GROTA, LG
用两种不同的抗血清，一种是仅结合褪黑素(M)的血清，另一种是结合M和N-乙酰5-羟色胺(NAS)的血清，用荧光和过氧化物酶标记的双抗体免疫组织化学方法对大鼠脑组织和哈德氏腺进行了研究。M定位于视网膜外核层、视神经、视交叉、视交叉束、视交叉上核和哈德氏腺。NAS分布于小脑颗粒层、三叉神经根脊束及脑桥和脊髓网状结构。讨论了小脑和脑干NAS与脑兴奋性的关系以及M在视觉通路、视交叉上核和哈德氏腺的定位与光依赖昼夜节律调节的关系。
Using 2 different antisera, 1 binding only melatonin (M) and the other binding M and N-acetylserotonin (NAS), brain tissue and the Harderian gland were investigated [in rats] using fluorescence and peroxidase labeled double antibody immunohistology. M was identified in the outer nuclear layer of the retina, in the optic nerves, chiasma and the tracts, suprachiasmatic nucleus and the Harderian gland. NAS was found in the granule layer of the cerebellum, spinal tract of the trigeminal roots and the pontal and spinal reticular formation. The possible relationship of cerebellar and brain stem NAS to brain excitability and localization of M in the visual pathway, suprachiasmatic nucleus and Harderian gland in the relation to the regulation of light dependent circadian periodicity is discussed.