Changes in the bacterial populations of the highly alkaline saline soil of the former lake Texcoco (Mexico) following flooding
Changes in the bacterial populations of the highly alkaline saline soil of the former lake Texcoco (Mexico) following flooding
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DOI：
10.1007/s00792-009-0244-4
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发表时间：
2009-04
期刊：
影响因子：
2.9
通讯作者：
César Valenzuela-Encinas;I. Neria-González;R. Alcántara-Hernández;I. Estrada-Alvarado;F. J. Zavala-Díaz de la Serna;L. Dendooven;R. Marsch
中科院分区：
文献类型：
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作者：
César Valenzuela-Encinas;I. Neria-González;R. Alcántara-Hernández;I. Estrada-Alvarado;F. J. Zavala-Díaz de la Serna;L. Dendooven;R. Marsch
淹没极端碱性盐碱地会降低土壤的碱度和盐度，从而改变细菌数量。利用通用细菌寡核苷酸引物，对ec1.7 dS m−1和pH 7.80（低土壤）、ec56 dS m−1和pH 10.11（中等土壤）和ec159 dS m−1和pH 10.02（高土壤）3种不同电解电导率的土壤（EC）建立了细菌16S rDNA文库，并对463个克隆16S rDNA序列进行了系统发育分析。变形菌门、放线菌门、酸性菌门、蓝藻门、拟杆菌门、厚壁菌门和绿藻门的文库比例和克隆鉴定表明，细菌群落存在差异。在这三个地点均发现了变形菌群α-亚群和γ-亚群的根瘤菌目、红杆菌目和黄菌目的种属。高土中有红螺旋菌门、鞘菌门、梭菌门、梭菌门和Caldilineales，中土中有鞘菌门、burkholderales和Pseudomonadales，低土中有黏液球菌门，中、低土中有放线菌门。结果表明，中等土壤中细菌种类多样性最大，高、低土壤中均有细菌存在。
Flooding an extreme alkaline-saline soil decreased alkalinity and salinity, which will change the bacterial populations. Bacterial 16S rDNA libraries were generated of three soils with different electrolytic conductivity (EC), i.e. soil with EC 1.7 dS m−1and pH 7.80 (LOW soil), with EC 56 dS m−1and pH 10.11 (MEDIUM soil) and with EC 159 dS m−1and pH 10.02 (HIGH soil), using universal bacterial oligonucleotide primers, and 463 clone 16S rDNA sequences were analyzed phylogenetically. Library proportions and clone identification of the phylaProteobacteria,Actinobacteria,Acidobacteria,Cyanobacteria,Bacteroidetes,FirmicutesandCloroflexishowed that the bacterial communities were different. Species and genera of the Rhizobiales, Rhodobacterales and Xanthomonadales orders of the α- and γ-subdivision ofProteobacteriawere found at the three sites. Species and genera of the Rhodospirillales, Sphingobacteriales, Clostridiales, Oscillatoriales and Caldilineales were found only in the HIGH soil, Sphingomonadales, Burkholderiales and Pseudomonadales in the MEDIUM soil, Myxococcales in the LOW soil, and Actinomycetales in the MEDIUM and LOW soils. It was found that the largest diversity at the order and species level was found in the MEDIUM soil as bacteria of both the HIGH and LOW soils were found in it.