Genotoxicity of nanoscale zerovalent iron particles in tobacco BY-2 cells
Genotoxicity of nanoscale zerovalent iron particles in tobacco BY-2 cells
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DOI：
10.1007/s13237-019-00294-z
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发表时间：
2019-08
期刊：
影响因子：
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通讯作者：
Ilika Ghosh;A. Sadhu;Y. Moriyasu;Maumita Bandyopadhyay;A. Mukherjee
中科院分区：
文献类型：
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作者：
Ilika Ghosh;A. Sadhu;Y. Moriyasu;Maumita Bandyopadhyay;A. Mukherjee
纳米修复通过直接注射nZVI到受污染的水或土壤中产生了他们的释放到环境中，促进与植物系统的相互作用。现有的报告显示缺乏关于nZVI在植物中的遗传毒性的信息。在这项研究中，nZVI的细胞遗传毒性潜力进行了评估，在烟草BY-2细胞处理24小时。对5至500 µg/mL的广泛浓度范围的nZVI进行了细胞毒性试验，对于其他试验，使用了20 µg/mL的最大浓度。通过TTC和伊文思蓝试验评估的细胞毒性显示，用最低浓度的nZVI（5 µg/mL）处理的细胞中细胞死亡最多。彗星试验证实了低浓度下的高DNA损伤，这证实了普鲁士蓝染色观察到的nZVI的高积累。ROS的定性和定量估计证实了超氧阴离子，羟基，过氧自由基和过氧化氢的积累后，处理低浓度的nZVI。总之，本研究证实了ROS介导的nZVI细胞毒性和遗传毒性，并揭示了nZVI安全性评价以及其他生态毒理学相关纳米材料毒性测试的进一步前景。
Nanoremediation by direct injection of nZVI into contaminated water or soil has engendered their liberation into the environment facilitating interaction with plant systems. Available reports display a scarcity of information on the genotoxicity of nZVI in plants. In this study, the cyto-genotoxic potential of nZVI was evaluated in tobacco BY-2 cells treated for 24 h. A wide range of concentrations from 5 to 500 µg/mL of nZVI were tested for cytotoxicity and for other assays a maximum concentration of 20 µg/mL was used. Cytotoxicity assessed by TTC and Evans Blue tests showed maximum cell death in cells treated with the lowest concentration of nZVI (5 µg/mL). Comet assay confirmed high DNA damage at low concentrations, which corroborated with high accumulation of nZVI as observed by Prussian blue staining. Qualitative and quantitative estimation of ROS confirmed the accumulation of superoxide anions, hydroxyl, peroxyl radicals and hydrogen peroxide upon treatment with low concentrations of nZVI. Taken together, this study confirmed ROS mediated cytotoxicity and genotoxicity of nZVI and unravels further prospects in the safety evaluation of nZVI as well as the toxicity testing of other nanomaterials of ecotoxicological relevance.