Treatment and control of mycoplasma contamination in Plasmodium falciparum culture
Treatment and control of mycoplasma contamination in Plasmodium falciparum culture
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DOI：
10.1007/s00436-008-1181-3
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发表时间：
2008-12-01
影响因子：
2
通讯作者：
Srivastava, Kumkum
中科院分区：
文献类型：
--
作者：
Singh, Shubhra;Puri, S. K.;Srivastava, Kumkum
测定了四种抗生素Plasmocin（大环内酯）、Biomyc-1、-2（四环素）和Biomyc-3以及支原体清除剂（喹诺酮衍生物）对恶性疟原虫培养物中支原体清除的比较效力。使用基于酶-PCR的支原体检测试剂盒检测支原体的存在，并在由经处理和未经处理的培养物制成的Giemsa染色涂片中确定疟原虫的存活。观察到Biomyc-1和Biomyc-2的组合在24小时内杀死疟疾寄生虫，而plasmocin和Biomyc-3导致疟疾寄生虫在6天的时间内缓慢死亡。从恶性疟原虫培养物中观察到唯一不杀死疟原虫和根除支原体的化合物是MRA。
A comparative efficacy of four antibiotics, plasmocin (macrolid), Biomyc-1, -2, (tetracycline), and Biomyc-3, and Mycoplasma Removing Agent (quinolone derivatives) was determined for elimination of mycoplasma from Plasmodium falciparum culture. Presence of mycoplasma was detected using enzyme-PCR-based mycoplasma detection kit and survival of malaria parasite was determined in Giemsa's stained smear made from treated and untreated cultures. It was observed that a combination of Biomyc-1 and -2 killed malaria parasites within 24 h, whereas plasmocin and Biomyc-3 caused slow death of malaria parasite stretched over a period of 6 days. The only compound which did not kill malaria parasite and eradicated mycoplasma from P. falciparum culture was observed to be MRA.