Computational genomic identification and functional reconstitution of plant natural product biosynthetic pathways.
Computational genomic identification and functional reconstitution of plant natural product biosynthetic pathways.
复制标题
DOI：
10.1039/c6np00035e
复制
发表时间：
2016-08-27
影响因子：
11.9
通讯作者：
Osbourn A
中科院分区：
文献类型：
--
作者：
Medema MH;Osbourn A
过去十年，关于植物天然产物生物合成途径的基因组基础首次取得重大发现。覆盖物：2003年至2016年过去十年，人们首次发现了植物天然产物生物合成途径基因组基础的重大发现。已经开发了四种关键的计算驱动策略来识别这样的途径，其利用参与产生植物天然产物的基因的物理聚类、共表达、进化共现和表观基因组共调控。在这里，我们将讨论如何使用这些方法来发现植物生物合成途径编码的染色体簇和非簇基因。此外，我们将讨论优先考虑植物基因簇进行实验表征的机会，并以前瞻性的视角结束合成生物学技术如何使用各种遗传系统有效地重建候选途径。
The last decade has seen the first major discoveries regarding the genomic basis of plant natural product biosynthetic pathways. Covering: 2003 to 2016 The last decade has seen the first major discoveries regarding the genomic basis of plant natural product biosynthetic pathways. Four key computationally driven strategies have been developed to identify such pathways, which make use of physical clustering, co-expression, evolutionary co-occurrence and epigenomic co-regulation of the genes involved in producing a plant natural product. Here, we discuss how these approaches can be used for the discovery of plant biosynthetic pathways encoded by both chromosomally clustered and non-clustered genes. Additionally, we will discuss opportunities to prioritize plant gene clusters for experimental characterization, and end with a forward-looking perspective on how synthetic biology technologies will allow effective functional reconstitution of candidate pathways using a variety of genetic systems.