Development of a molecular‐based index for assessing iron status in bloom‐forming pennate diatoms
Development of a molecular‐based index for assessing iron status in bloom‐forming pennate diatoms
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开发基于分子的指数，用于评估开花羽状硅藻中的铁状态
DOI：
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发表时间：
2017
影响因子：
2.9
通讯作者：
Robert H. Lampe
中科院分区：
文献类型：
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作者：
A. Marchetti;Carly M. Moreno;N. Cohen;Irina V. Oleinikov;K. deLong;B. Twining;E. Virginia Armbrust;Robert H. Lampe
铁的供应限制了世界海洋大片区域的初级生产力。确定浮游植物的铁状况对于理解调节其生长和生态的因素至关重要。我们开发了一种不依赖于孵育的、基于分子的方法来评估硅藻群落特定成员的铁营养状况，最初专注于生态学上重要的羽状硅藻伪菱形藻。通过比较转录组学方法，我们确定了两个基因，以高保真度跟踪假菱形藻的铁状态。第一个基因，铁蛋白（FTN），编码在铁充足的条件下诱导的高度专门化的铁储存蛋白。第二个基因ISIP2a编码在铁限制条件下诱导的铁浓缩蛋白。在海洋硅藻Granii（Hasle）Hasle中，这些基因的转录丰度与铁可用性的变化直接相关，在铁充足条件下FTN转录丰度增加，在铁限制条件下ISIP2a转录丰度增加。所得ISIP2a：FTN转录物比率反映细胞的铁状态，其中高比率指示铁限制。在阿拉斯加湾的低铁沃茨和加州上升流区的可变铁沃茨进行的铁生长微宇宙实验中收集的现场样品验证了我们提出的伪菱形铁限制指数的有效性，该指数可用于确定原位铁状态，并进一步发展为其他生态重要的硅藻。
Iron availability limits primary productivity in large areas of the world's oceans. Ascertaining the iron status of phytoplankton is essential for understanding the factors regulating their growth and ecology. We developed an incubation‐independent, molecular‐based approach to assess the iron nutritional status of specific members of the diatom community, initially focusing on the ecologically important pennate diatom Pseudo‐nitzschia. Through a comparative transcriptomic approach, we identified two genes that track the iron status of Pseudo‐nitzschia with high fidelity. The first gene, ferritin (FTN), encodes for the highly specialized iron storage protein induced under iron‐replete conditions. The second gene, ISIP2a, encodes an iron‐concentrating protein induced under iron‐limiting conditions. In the oceanic diatom Pseudo‐nitzschia granii (Hasle) Hasle, transcript abundance of these genes directly relates to changes in iron availability, with increased FTN transcript abundance under iron‐replete conditions and increased ISIP2a transcript abundance under iron‐limiting conditions. The resulting ISIP2a:FTN transcript ratio reflects the iron status of cells, where a high ratio indicates iron limitation. Field samples collected from iron grow‐out microcosm experiments conducted in low iron waters of the Gulf of Alaska and variable iron waters in the California upwelling zone verify the validity of our proposed Pseudo‐nitzschia Iron Limitation Index, which can be used to ascertain in situ iron status and further developed for other ecologically important diatoms.
影响因子：
4.4
作者：
Lommer M;Roy AS;Schilhabel M;Schreiber S;Rosenstiel P;LaRoche J
通讯作者：
LaRoche J