Induction of immune response in goats immunized with Brucella melitensis 16M DNA
Induction of immune response in goats immunized with Brucella melitensis 16M DNA
复制标题
用羊布鲁氏菌 16M DNA 免疫的山羊中诱导免疫反应
DOI：
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
V. K. Vihan
中科院分区：
文献类型：
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作者：
V. Gupta;P. K. Rout;T. Chandrasekhar;V. K. Vihan
采用不同剂量率（0.5mg、1.0mg和1.5mg/只）对3组山羊进行肌内直接注射B.melifensis DNA，每组4只山羊。两组作为对照（1 组阳性，1 组阴性）。每隔 3 周进行 3 次注射。每周（最多 5 周）分析体液免疫反应，并在最后一次 DNA 注射后第 15 天和第 30 天分析淋巴增殖反应。接种后 5 周测量的体液反应表明，注射 1.0mg DNA 会产生长效 IgG 反应。通过研究 T 细胞反应来分析 DNA 诱导的细胞免疫反应。 0.5 长 DNA 注射和 1.5 mg DNA 注射均导致对羊种布鲁氏菌提取物的低特异性 T 细胞增殖，而 1.0 mg DNA 接种的动物则表现出相对较高的 T 细胞增殖。在接种后 30 天也发现了这种特异性诱导的增殖反应。
The direct injections of B.melifensis DNA were given by intramuscular rout at different dose rates (0.5mg, 1.0mg and 1.5 mg/animal) to 3 groups of goats, each group comprised 4 goats each. Two groups were kept as control (1 positive and 1 negative). Three injections at 3-week intervals were given. The humoral immune response was analyzed every week (up to 5 week) and Iymphoproliferative response was analysed at 15th and 30th day after the last DNA injection. Humoral response measured 5 weeks post inoculation indicated that 1.0mg DNA injection leads to the generation of long-lived IgG responses. Cellular immune response induced by the DNA was analyzed by studying T-cell response. Both 0.5 long and 1.5mg DNA injection resulted in low specific T-cell proliferation in response to the Brucella melitensis extracts while 1.0 mg DNA inoculated animals showed comparatively high T-cell proliferation. This specific induced proliferative response was also found at 30 day postinoculation.