Identification of four Engrailed genes in the Japanese lamprey, Lethenteron japonicum
Identification of four Engrailed genes in the Japanese lamprey, Lethenteron japonicum
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DOI：
10.1002/dvdy.21552
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发表时间：
2008-06-01
影响因子：
2.5
通讯作者：
Tanaka, Mikiko
中科院分区：
文献类型：
--
作者：
Matsuura, Manami;Nishihara, Hidenori;Tanaka, Mikiko
我们已经从日本七鳃鳗，Lethenteron japonicum，一个无颌动物，占据了一个关键的基因位置之间的cephalocordates和gnathostomes的Engrailed基因的四个同源物。我们将这四个基因命名为LjEngrailedA、LjEngrailedB、LjEngrailedC和LjEngrailedD。LjEngrailedA、LjEngrailedB和LjEngrailedD在中枢神经系统的假定中脑-后脑边界区域中共享主要表达结构域，尽管它们的表达水平和时间不同。另一方面，LjEngrailedC转录本存在于体壁的咽外胚层和腹外胚层。此外，LjEngrailedA在鳃上肌前体的腹侧表达。LjEngrailedD成绩单被认为是中胚层细胞的下颌弓，后来在一组细胞负责形成的上唇，下唇，和软腭。我们的研究结果为这些结构的进化提供了线索，也为Engrailed基因的复制事件提供了可能的场景。
We have isolated four homologs of Engrailed genes from the Japanese lamprey, Lethenteron japonicum, an agnathan that occupies a critical phylogenic position between cephalochordates and gnathostomes. We named these four genes LjEngrailedA, LjEngrailedB, LjEngrailedC, and LjEngrailedD. LjEngrailedA, LjEngrailedB, and LjEngrailedD share a major expression domain in the presumptive midbrain-hindbrain boundary region of the central nervous system, although their levels and timing of expression differed. On the other hand, LjEngrailedC transcripts were in the pharyngeal ectoderm and the ventral ectoderm of the body wall. In addition, LjEngrailedA was expressed in the ventral side of the epibranchial muscle precursors. LjEngrailedD transcripts were seen in the mesodermal cells of the mandibular arch and later in a group of cells responsible for the formation of the upper lip, lower lip, and velum. Our results provide clues to the evolution of these structures as well as a possible scenario for duplication events of Engrailed genes.