Differential perikaryal localization in rats of D1 and D2 dopamine receptors on striatal projection neuron types identified by retrograde labeling
Differential perikaryal localization in rats of D1 and D2 dopamine receptors on striatal projection neuron types identified by retrograde labeling
复制标题
通过逆行标记鉴定纹状体投射神经元类型上 D1 和 D2 多巴胺受体的大鼠核周差异定位
DOI：
10.1016/j.jchemneu.2006.07.001
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发表时间：
2006-12-01
影响因子：
2.8
通讯作者：
Reiner, Anton
中科院分区：
文献类型：
--
作者：
Deng, Yun-Ping;Lei, Wan-Long;Reiner, Anton
D1和D2多巴胺受体的纹状体投射神经元类型的本地化一直存在争议，一些数据有利于隔离D1直接通路神经元（含P物质）和D2间接通路神经元（脑啡肽），和其他报告显着的D1和D2在个别投射神经元类型的共定位。在本研究中，我们使用亚型特异性抗体对D1和D2和共聚焦激光扫描显微镜，以确定其perikaryal定位在纹状体一般，并在直接和间接通路神经元perikarya定义的逆行标记特别。我们发现，在大鼠纹状体NeuN免疫标记的胞体中，49.5%的细胞可检测到D1，61.6%的细胞可检测到D2，这意味着至少15-20%的D1+神经元必须具有D2，反之亦然。其次，我们用罗丹明葡聚糖胺3 kDa（RDA 3 k）逆行标记了来自外苍白球（GPe）、内苍白球（GPi）或黑质的神经元胞体。我们发现，92%的黑质标记的胞体和96%的GPi标记的胞体免疫标记为D1，但只有23%的GPe标记的胞体免疫标记为D1。由于直接通路神经元（纹状体-黑质和纹状体-GPi）具有到GPe的侧支投射，因此可能许多从GPe逆行标记的D1+纹状体胞体是直接通路神经元。约96%的从GPe逆行标记的胞体被免疫标记为D2，而约40%的从GPi逆行标记的胞体和44%的从黑质逆行标记的胞体被免疫标记为D2。这些研究结果表明：（1）虽然许多纹状体-GPi/SN神经元具有D1和D2，但大多数主要或仅具有D1;（2）绝大多数纹状体-GPe神经元主要或仅具有D2。(c)2006 Elsevier B. V.保留所有权利。
The localization of D1 and D2 dopamine receptors to striatal projection neuron types has been controversial, with some data favoring segregation of D1 to direct pathway neurons (substance P-containing) and D2 to indirect pathway neurons (enkephalinergic), and others reporting significant colocalization of D1 and D2 on individual projection neuron types. In the present study, we used subtype-specific antibodies against D1 and D2 and confocal laser scanning microscopy to determine their perikaryal localization in striatum in general, and in direct and indirect pathway neuron perikarya defined by retrograde labeling in particular. We found that D1 in rat was detectable on 49.5% of NeuN-immunolabeled striatal perikarya, and D2 on 61.6% of NeuN-immunolabeled perikarya, implying that at least 15-20% of D1+ neurons must possess D2 and vice versa. Secondly, we retrogradely labeled neuronal perikarya from the external globus pallidus (GPe), internal globus pallidus (GPi) or substantia nigra ;with rhodamine dextran amine 3 kDa (RDA3k). We found that 92% of perikarya labeled from nigra and 96% of perikarya labeled from GPi immunolabeled for D1, but only 23% of perikarya labeled from GPe immunolabeled for D1. Since direct pathway neurons (striato-nigral and striato-GPi) have a collateral projection to GPe, it is possible that many of the D1+ striatal perikarya retrogradely labeled from GPe were direct pathway neurons. About 96% of perikarya retrogradely labeled from GPe were immunolabeled for D2, while about 40% of those retrogradely labeled from GPi and 44% of those retrogradely labeled from nigra immunolabeled for D2. These findings suggest that: (1) while many striato-GPi/SN neurons possess D1 and D2, the majority mainly or exclusively possess D1 and (2) the vast majority of striato-GPe neurons mainly or exclusively possess D2. (c) 2006 Elsevier B.V. All rights reserved.