An ESP1/PDS1 complex regulates loss of sister chromatid cohesion at the metaphase to anaphase transition in yeast
An ESP1/PDS1 complex regulates loss of sister chromatid cohesion at the metaphase to anaphase transition in yeast
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DOI：
10.1016/s0092-8674(00)81211-8
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发表时间：
1998-06-12
期刊：
影响因子：
64.5
通讯作者：
Nasmyth, K
中科院分区：
文献类型：
--
作者：
Ciosk, R;Zachariae, W;Nasmyth, K
在G2和M期姐妹染色单体之间的凝聚力取决于“凝聚素”蛋白Scc1p（Mcd1p）。在中期到后期过渡的凝聚力的损失是伴随着Scc1p的从染色单体的解离，这取决于蛋白水解的Pds1p介导的泛素蛋白连接酶称为后期促进复合物（APC）。我们表明，破坏Pds1p是APC的唯一作用，触发Scc1p的解离从染色单体和Pds1p形成一个稳定的复合物与180 kDa的蛋白质称为Esp1p，这是必不可少的Scc1p从姐妹染色单体的解离和分离。我们认为APC促进姐妹分离不是通过破坏粘着蛋白，而是通过从其抑制剂Pds1p中释放“姐妹分离”Esp1蛋白。
Cohesion between sister chromatids during G2 and M phases depends on the "cohesin" protein Scc1p (Mcd1p). Loss of cohesion at the metaphase to anaphase transition is accompanied by Scc1p's dissociation from chromatids, which depends on proteolysis of Pds1p mediated by a ubiquitin protein ligase called the anaphase promoting complex (APC). We show that destruction of Pds1p is the APC's sole role in triggering Scc1p's dissociation from chromatids and that Pds1p forms a stable complex with a 180 kDa protein called Esp1p, which is essential for the dissociation of Scc1p from sister chromatids and for their separation. We propose that the APC promotes sister separation not by destroying cohesins but instead by liberating the "sister-separating" Esp1 protein from its inhibitor Pds1p.