Sensitivity Comparison of Macro- and Micro-Electrochemical Biosensors for Human Chorionic Gonadotropin (hCG) Biomarker Detection
Sensitivity Comparison of Macro- and Micro-Electrochemical Biosensors for Human Chorionic Gonadotropin (hCG) Biomarker Detection
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DOI：
10.1109/access.2019.2928132
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发表时间：
2019-01-01
期刊：
影响因子：
3.9
通讯作者：
Awan, Shakil A.
中科院分区：
文献类型：
--
作者：
Damiati, Samar;Haslam, Carrie;Awan, Shakil A.
选择性和灵敏度是电化学生物传感器设计和优化的重要指标。制造的免疫传感表面的效率可以容易地受到几个因素的影响，例如检测极限、非特异性结合和传感平台的类型。在这里，我们展示了宏观和微观尺寸的平面工作电极（4毫米和400 μ m直径，分别）的电化学行为和开发的生物传感器检测人绒毛膜促性腺激素（hCG），这是几种肿瘤的生物标志物的能力的影响。通过用连接体1-芘丁酸-N-羟基-琥珀酰亚胺酯（PANHS）修饰碳宏电极和微电极，并固定抗hCG抗体以特异性检测hCG蛋白，来构建所制造的丝网印刷传感器。通过循环伏安法（CV）和方波伏安法（SWV）对所开发的电极进行表征。每个免疫传感系统都表现出独特的电化学行为，这可能是由于颗粒在表面的排列。然而，发现与具有100 pg/mL的较低检测限的宏电极传感器相比，较小的微电极表面积显示出更高的灵敏度（1 pg/mL）。此外，拉曼光谱分析证实，与宏电极相比，微电极具有相对低的缺陷和无序密度。所提出的测定代表了一种有前途的方法，其对于分析物的特异性检测是高度有效的，并且可以被利用来靶向用于各种护理点诊断应用的生物标志物。
Selectivity and sensitivity are important figures of merit in the design and optimization of electrochemical biosensors. The efficiency of a fabricated immunosensing surface can easily be influenced by several factors, such as the detection limit, non-specific binding, and type of sensing platform. Here, we demonstrate the effects of macro-and micro-sized planner working electrodes (4 mm and 400 mu m diameter, respectively) on electrochemical behavior and the ability of the developed biosensor to detect human chorionic gonadotropin (hCG), which is a biomarker of several tumors. The fabricated screen-printed sensor was constructed by modifying the carbon macro- and micro-electrodes with a linker, 1-pyrenebutyric acid-N-hydroxy-succinimide ester (PANHS), and immobilization of anti-hCG antibodies to specifically detect the hCG protein. The characterization of the developed electrodes was performed by cyclic voltammetry (CV) and squarewave voltammetry (SWV). Each immunosensing system showed unique electrochemical behavior, which might be attributed to the arrangement of particles on the surface. However, a smaller micro-electrode surface area was found to show higher sensitivity (1 pg/mL) compared to the macro-electrode sensor with a lower detection limit of 100 pg/mL. Further, Raman spectroscopy analysis confirmed that the micro-electrode had a relatively low density of defects and disorder compared to the macro-electrode. The proposed assay represents a promising approach that is highly effective for specific detection of an analyte and can be exploited to target biomarkers for a variety of point-of-care diagnostic applications.