Brain sources of inhibitory input to the rat rostral ventrolateral medulla
Brain sources of inhibitory input to the rat rostral ventrolateral medulla
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DOI：
10.1002/cne.23175
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发表时间：
2013-01-01
影响因子：
2.5
通讯作者：
Goodchild, Ann K.
中科院分区：
文献类型：
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作者：
Bowman, Belinda R.;Kumar, Natasha N.;Goodchild, Ann K.
延髓头端腹外侧区(RVLM)含有对心血管、呼吸、代谢和运动控制至关重要的神经元。这些神经元的活动由来自多个确定的大脑区域的输入控制；然而，这些输入的神经化学在很大程度上是未知的。γ-氨基丁酸(GABA)和脑啡肽能强直地抑制RVLM内的神经元。本研究的目的是确定向大鼠RVLM提供GABA能或脑啡肽能输入的所有脑区。用原位杂交法检测RVLM逆行输送的霍乱毒素B免疫反应阳性神经元谷氨酸脱羧酶(GAD67)或前脑啡肽原(PPE)mRNA的表达。孤束核(NTS，占CTB阳性神经元的6%)、最后区(AP，8%)、延髓尾侧腹外侧区(17%)、中缝中线(40%)、中脑导水管周围灰质腹外侧区(VLPAG，15%)、下丘脑外侧区(LHA，25%)、杏仁中央核(CEA，77%)、杏仁核豆状核(Slea，86%)、前连合后肢间质核(IPAC，56%)、纹状终末核(BNST，59%)、杏仁中央核(CEA，77%)、杏仁核豆状核(Slea，86%)、前连合后肢间质核(IPAC，56%)、纹状终末床核(BNST，59%)、杏仁核中央核(CEA，77%)、杏仁核豆状核(Slea，86%)、前连合后肢间质核(IPAC，56%)、纹状终末核(BNST，59%)、杏仁中央核(CEA，77%)、杏仁核豆状核(Slea，86%)、前连合后肢间质核(IPAC，56%)、终纹终核(BNST，59%)、杏仁中央核(CEA，77%)、杏仁核豆状核(Slea，86%)、前连合后肢间内侧视前区(Mpa，53%)。在CTB-ir神经元中，PPE基因在NTS(占CTB-ir神经元的14%)、中线中缝(26%)、LHA(22%)、透明带(Zi，15%)、CEA(5%)、室旁核(PVN，13%)、Slea(66%)和MPa(26%)有表达。因此，有限的脑区为RVLM提供GABA能和/或脑啡肽能输入。多条神经化学上不同的通路起源于这些投射到RVLM的大脑区域。J.Comp.神经。521：213232,2013年。(C)2012年威利期刊公司。
The rostral ventrolateral medulla (RVLM) contains neurons critical for cardiovascular, respiratory, metabolic, and motor control. The activity of these neurons is controlled by inputs from multiple identified brain regions; however, the neurochemistry of these inputs is largely unknown. Gamma-aminobutyric acid (GABA) and enkephalin tonically inhibit neurons within the RVLM. The aim of this study was to identify all brain regions that provide GABAergic or enkephalinergic input to the rat RVLM. Neurons immunoreactive for cholera toxin B (CTB-ir), retrogradely transported from the RVLM, were assessed for expression of glutamic acid decarboxylase (GAD67) or preproenkephalin (PPE) mRNA using in situ hybridization. GAD67 mRNA was expressed in CTB-ir neurons in the following regions: the nucleus of the solitary tract (NTS, 6% of CTB-ir neurons), area postrema (AP, 8%), caudal ventrolateral medulla (17%), midline raphe (40%), ventrolateral periaqueductal gray (VLPAG, 15%), lateral hypothalamic area (LHA, 25%), central nucleus of the amygdala (CeA, 77%), sublenticular extended amygdala (SLEA, 86%), interstitial nucleus of the posterior limb of the anterior commissure (IPAC, 56%), bed nucleus of the stria terminals (BNST, 59%), and medial preoptic area (MPA, 53%). PPE mRNA was expressed in CTB-ir neurons in the following regions: the NTS (14% of CTB-ir neurons), midline raphe (26%), LHA (22%), zona incerta (ZI, 15%), CeA (5%), paraventricular nucleus (PVN, 13%), SLEA (66%), and MPA (26%). Thus, limited brain regions contribute GABAergic and/or enkephalinergic input to the RVLM. Multiple neurochemically distinct pathways originate from these brain regions projecting to the RVLM. J. Comp. Neurol. 521:213232, 2013. (C) 2012 Wiley Periodicals, Inc.