Beyond the random coil: stochastic conformational switching in intrinsically disordered proteins.
Beyond the random coil: stochastic conformational switching in intrinsically disordered proteins.
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DOI：
10.1016/j.str.2011.01.011
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发表时间：
2011-04-13
期刊：
影响因子：
5.7
通讯作者：
Bowen, Mark E.
中科院分区：
文献类型：
--
作者：
Choi, Ucheor B.;McCann, James J.;Weninger, Keith R.;Bowen, Mark E.
固有无序蛋白(IDPs)参与关键的细胞功能，利用其天然状态的灵活性和快速构象波动。关于国内流离失所者的自然状态的有限信息可以通过对许多异质分子进行平均来获得，这在系综方法中是不可避免的。我们使用单分子荧光来表征五个被其他技术证实为无序的突触蛋白的自然状态构象动力学。SNAP-25、Synaptobrevin和Complin三种蛋白质的构象动力学可以用简单的半柔性聚合物模型来描述。令人惊讶的是，两种蛋白质，神经连接素和NMDAR-2B谷氨酸受体，被观察到在不同的构象状态之间随机切换，尽管事实是，它们在其他指标上看起来本质上是无序的。在这些蛋白质中发现的啤酒花状分子内扩散被认为是定义了一类以前没有被识别的内分泌蛋白的功能。
Intrinsically disordered proteins (IDPs) participate in critical cellular functions that exploit the flexibility and rapid conformational fluctuations of their native state. Limited information about the native state of IDPs can be gained by the averaging over many heterogeneous molecules that is unavoidable in ensemble approaches. We used single molecule fluorescence to characterize native state conformational dynamics in five synaptic proteins confirmed to be disordered by other techniques. For three of the proteins, SNAP-25, synaptobrevin and complexin, their conformational dynamics could be described with a simple semi-flexible polymer model. Surprisingly, two proteins, neuroligin and the NMDAR-2B glutamate receptor, were observed to stochastically switch among distinct conformational states despite the fact that they appeared intrinsically disordered by other measures. The hop-like intramolecular diffusion found in these proteins is suggested to define a class of functionality previously unrecognized for IDPs.
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影响因子：
16.2
作者：
Hayashi, Takashi;Thomas, Gareth M.;Huganir, Richard L.
通讯作者：
Huganir, Richard L.
影响因子：
6.8
作者：
Eliezer, David
通讯作者：
Eliezer, David
DOI：
10.1073/pnas.0911107107
发表时间：
2010-05-04
影响因子：
11.1
作者：
Mao, Albert H.;Crick, Scott L.;Pappu, Rohit V.
通讯作者：
Pappu, Rohit V.
DOI：
10.1073/pnas.0508584102
发表时间：
2005-11-29
影响因子：
11.1
作者：
Laurence, TA;Kong, XX;Weiss, S
通讯作者：
Weiss, S
DOI：
10.1073/pnas.090104997
发表时间：
2000-05-09
影响因子：
11.1
作者：
Deniz, AA;Laurence, TA;Weiss, S
通讯作者：
Weiss, S