RECONSTITUTION OF BIOCHEMICALLY ALTERED NUCLEAR-PORES - TRANSPORT CAN BE ELIMINATED AND RESTORED
RECONSTITUTION OF BIOCHEMICALLY ALTERED NUCLEAR-PORES - TRANSPORT CAN BE ELIMINATED AND RESTORED
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DOI：
10.1016/0092-8674(90)90712-n
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发表时间：
1990-01-12
期刊：
影响因子：
64.5
通讯作者：
FORBES, DJ
中科院分区：
文献类型：
--
作者：
FINLAY, DR;FORBES, DJ
在核组装提取物中构建了生化改变的核孔，特别是缺乏含n -乙酰氨基葡萄糖的孔蛋白，以便赋予这些蛋白功能。耗尽的孔不结合核信号序列或主动输入核蛋白，但它们具有扩散功能。这些缺陷可以通过与读取的爪蟾孔糖蛋白组装来完全修复。引人注目的是，分离的大鼠孔糖蛋白也能恢复运输。电镜显示枯竭的孔隙形态基本正常。因此，孔糖蛋白对于核膜、孔的主要结构或孔扩散通道的组装是不需要的。相反，它们对于活性蛋白的导入是必不可少的，而且出乎意料的是，它们对于构建信号序列识别所必需的部分孔是必不可少的。
Biochemically altered nuclear pores specifically lacking the N-acetylglucosamine-bearing pore proteins were constructed in a nuclear assembly extract in order to assign function to these proteins. The depleted pores do not bind nuclear signal sequences or actively import nuclear proteins, but they are functional for diffusion. These defects can be fully repaired by assembly with readded Xenopus pore glycoproteins. Strikingly, isolated rat pore glycoproteins also restore transport. Electron microscopy reveals that depleted pores have largely normal morphology. Thus, the pore glycoproteins are not required for assembly of the nuclear envelope, the major structures of the pore, or a pore diffusional channel. Instead, they are essential for active protein import and, unexpectedly, for construction of the part of the pore necessary for signal sequence recognition.