EUCOMM - the European Conditional Mouse Mutagenesis Program
EUCOMM - the European Conditional Mouse Mutagenesis Program
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DOI：
10.1093/bfgp/elm022
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发表时间：
2007-09-01
期刊：
影响因子：
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通讯作者：
Wurst, Wolfgang
中科院分区：
文献类型：
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作者：
Friedel, Roland H.;Seisenberger, Claudia;Wurst, Wolfgang
哺乳动物基因组的功能分析是生物医学科学家面临的巨大挑战。为了促进这一努力，欧洲条件小鼠诱变计划（EUCOMM）的目标是在小鼠胚胎干细胞（ES）中通过基因捕获产生多达12000个突变和通过基因靶向产生多达8000个突变。这些突变可以呈现为条件等位基因，允许Cre重组酶以时间和组织特异性方式介导的基因功能破坏。此外，EUCOMM程序将从EUCOMM资源生成多达320条鼠标线和多达20条新的Cre驱动程序鼠标线。EUCOMM的载体、突变ES细胞系和突变小鼠资源将向科学界开放。EUCOMM将成为创建全球小鼠突变资源的国际努力的基石之一。
Functional analysis of the mammalian genome is an enormous challenge for biomedical scientists. To facilitate this endeavour, the European Conditional Mouse Mutagenesis Program (EUCOMM) aims at generating up to 12000 mutations by gene trapping and up to 8000 mutations by gene targeting in mouse embryonic stem (ES) cells. These mutations can be rendered into conditional alleles, allowing Cre recombinase-mediated disruption of gene function in a time- and tissue-specific manner. Furthermore, the EUCOMM program will generate up to 320 mouse lines from the EUCOMM resource and up to 20 new Cre driver mouse lines. The EUCOMM resource of vectors, mutant ES cell lines and mutant mice will be openly available to the scientific community. EUCOMM will be one of the cornerstones of an international effort to create a global mouse mutant resource.