Compatibility between haemagglutinin and neuraminidase drives the recent emergence of novel clade 2.3.4.4 H5Nx avian influenza viruses in China
Compatibility between haemagglutinin and neuraminidase drives the recent emergence of novel clade 2.3.4.4 H5Nx avian influenza viruses in China
复制标题
血凝素和神经氨酸酶之间的相容性推动了新型分支 2.3.4.4 H5Nx 禽流感病毒最近在中国的出现。
DOI：
10.1111/tbed.12949
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发表时间：
2018-12-01
影响因子：
4.3
通讯作者：
Liu, Xiufan
中科院分区：
文献类型：
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作者：
Qin, Tao;Zhu, Jingjing;Liu, Xiufan
自2010年以来，高致病性禽流感（HPAI）H5亚型病毒与不同神经氨酸酶（NA）亚型之间的遗传相似性在中国野鸟和家禽中的流行率有所增加。HA基因从进化枝2.3.4略微进化到进化枝2.3.4.4，提出了新进化枝2.3.4.4 HA是否打破了与N1的平衡但与NAx匹配良好以驱动病毒流行的问题。为了阐明HA和NA之间的相容性在H5 Nx亚型流行中的作用，我们构建了10个重组病毒，其中进化枝2.3.4或进化枝2.3.4.4 HA基因与不同的NA（N1，N2和N8）基因相匹配，并评估病毒特性和致病性。进化枝2.3.4 HA和N1或进化枝2.3.4.4 HA和NAx之间的组合，但进化枝2.3.4.4 HA和N1或进化枝2.3.4 HA和NAx之间的组合，促进病毒在鸡和小鼠中的生长、NA活性、热稳定性、低pH稳定性和致病性。这些结果表明，进化枝2.3.4 HA/N1和进化枝2.3.4.4 HA/NAx表现出更好的匹配，这可能促进进化枝2.3.4 H5 N1 AIV（2010年之前）和进化枝2.3.4.4 H5 Nx AIV（2010年以来）在中国的流行分别增加。
Genetic reassortments between highly pathogenic avian influenza (HPAI) H5 subtype viruses with different neuraminidase (NA) subtypes have increased in prevalence since 2010 in wild birds and poultry from China. The HA gene slightly evolved from clade 2.3.4 to clade 2.3.4.4, raising the question of whether novel clade 2.3.4.4 HA broke the balance with N1 but is matched well with NAx to drive viral epidemics. To clarify the role of compatibility between HA and NA on the prevalence of H5Nx subtypes, we constructed 10 recombinant viruses in which the clade 2.3.4 or clade 2.3.4.4 HA genes were matched with different NA (N1, N2 and N8) genes and evaluated viral characteristics and pathogenicity. Combinations between clade 2.3.4 HA and N1 or between clade 2.3.4.4 HA and NAx, but not between clade 2.3.4.4 HA and N1, or between clade 2.3.4 HA and NAx, promoted viral growth, NA activity, thermostability, low-pH stability and pathogenicity in chicken and mice. These findings suggest that both clade 2.3.4 HA/N1 and clade 2.3.4.4 HA/NAx displayed a better match, which could promote the increased prevalence of clade 2.3.4 H5N1 AIV (prior to 2010) and clade 2.3.4.4 H5Nx AIV (since 2010) in China, respectively.