Unnatural base pairs mediate the site-specific incorporation of an unnatural hydrophobic component into RNA transcripts
Unnatural base pairs mediate the site-specific incorporation of an unnatural hydrophobic component into RNA transcripts
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DOI：
10.1016/j.bmcl.2004.02.072
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发表时间：
2004-05-17
影响因子：
2.7
通讯作者：
Yokoyama, S
中科院分区：
文献类型：
--
作者：
Endo, M;Mitsui, T;Yokoyama, S
通过非天然碱基对介导的T7转录，将疏水核苷酸类似物特异性地掺入到RNA中。用化学方法合成核苷酸类似物5-phenylethynyl-3-(beta-D-ribofuranosyl)pyridin-2-one 5-三磷酸(Ph-yTP)，然后用T7RNA聚合酶将其特异性地掺入模板中与2-氨基-6-(2-噻吩基)嘌呤(S表示)相对的RNA中。在茶碱结合的RNA适配子中引入Ph-y，其中内环中的尿苷被Ph-y取代，提高了适体的热稳定性。因此，这种非天然的核苷酸类似物对于稳定RNA的三级结构和RNA与其他分子之间的复合体是有用的。(C)2004爱思唯尔有限公司。保留所有权利。
Site-specific incorporation of a hydrophobic nucleotide analog into RNA, by T7 transcription mediated by unnatural base pairs, was developed. The nucleotide analog, 5-phenylethynyl-3-(beta-D-ribofuranosyl)pyridin-2-one 5-triphosphate (denoted by Ph-yTP), was chemically synthesized and then site-specifically incorporated by T7 RNA polymerase into RNA opposite the pairing partner, 2-amino-6-(2-thienyl)purine (denoted by s) in DNA templates. The introduction of Ph-y into a theophylline-binding RNA aptamer, in which a uridine in the internal loop was replaced by Ph-y, raised the thermal stability of the aptamer. Thus, this unnatural nucleotide analog would be useful for stabilizing RNA tertiary structures and complexes between RNA and other molecules. (C) 2004 Elsevier Ltd. All rights reserved.