Detection of endothelial progenitor cells in human skin wounds and its application for wound age determination
Detection of endothelial progenitor cells in human skin wounds and its application for wound age determination
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DOI：
10.1007/s00414-015-1181-7
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发表时间：
2015-09-01
影响因子：
2.1
通讯作者：
Kondo, Toshikazu
中科院分区：
文献类型：
--
作者：
Ishida, Yuko;Kimura, Akihiko;Kondo, Toshikazu
内皮祖细胞是一种新发现的细胞类型，是一种共表达干细胞标记物和血管内皮生长因子受体(Flk-1)的骨髓来源的祖细胞。本研究用抗CD34和抗Flk-1抗体对52个不同创面年龄(I组，0-1天；II组，2-6天；III组，7-14天；IV组，17-21天)的皮肤创面内皮祖细胞进行了双色免疫荧光分析。在小于1d的创面标本中，未检测到CD34(+)/Flk-1(+)内皮祖细胞。内皮祖细胞最初见于两天大的创面，随着创面年龄的延长，创面中内皮祖细胞的数量增加。在形态计量学分析中，Ⅲ组创面平均内皮祖细胞数量最多，尤其是7~12天的21个创面中有20个创面有20个内皮祖细胞，伤后14~21天的创面标本中有15个内皮祖细胞。这些观察至少表明>；20个EPC表明伤口的年龄为7-12天。综上所述，我们的观察表明内皮祖细胞的检测将有助于伤口年龄的确定。
Endothelial progenitor cells (EPCs), a newly identified cell type, are bone marrow-derived progenitor cells that co-express stem cell markers and vascular endothelial growth factor (VEGF) receptor (Flk-1). In this study, a double-color immunofluorescence analysis was carried out using anti-CD34 and anti-Flk-1 antibodies to examine the time-dependent appearance of EPCs, using 52 human skin wounds with different wound ages (Group I, 0-1 days; Group II, 2-6 days; Group III, 7-14 days; and Group IV, 17-21 days). In wound specimens with an age of less than one day, CD34(+)/Flk-1(+) EPCs were not detected. EPCs were initially observed in wounds aged two days, and their number was increased in lesions with advances in wound age. In morphometrical analysis, the average number of EPCs was the highest in the wounds of Group III. Especially, 20 out of 21 wounds aged 7-12 days had > 20 EPCs, and all wound samples with postinfliction intervals of 14-21 days had < 15 EPCs. These observations at least showed that > 20 EPCs would indicate a wound age of 7-12 days. Taken together, our observations indicate the detection of EPCs would be useful for wound age determination.