Regulation of blood-testis barrier by actin binding proteins and protein kinases.
Regulation of blood-testis barrier by actin binding proteins and protein kinases.
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DOI：
10.1530/rep-15-0463
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发表时间：
2016-03
期刊：
影响因子：
--
通讯作者：
Cheng CY
中科院分区：
文献类型：
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作者：
Li N;Tang EI;Cheng CY
血睾屏障（BTB）是睾丸中重要的超微结构，因为在啮齿动物和人类中，精子发生的开始与功能屏障的建立相一致。还注意到，在用药物如己烯雌酚或adjudin处理新生大鼠后，功能性BTB组装的延迟也延迟了精子的第一波。虽然BTB是最紧密的血液组织屏障之一，但它经历了广泛的重塑，特别是在上皮周期的第VIII阶段，以促进连接在克隆中的前细线期精母细胞穿过免疫屏障的运输。如果不能及时转运来自B型精原细胞的细线期前精母细胞，减数分裂将被阻止，导致精子发生。然而，自20世纪70年代的形态学研究以来，BTB的生物学和调控在很大程度上仍未被探索。然而，最近的研究对BTB的生物学有了新的认识。在此，我们批判性地评估这些发现中的一些，说明支持细胞BTB是由肌动蛋白结合蛋白（ABPs），可能支持非受体蛋白激酶，调节组织的肌动蛋白微丝束的网站。此外，微管（MT）为基础的细胞骨架也协同肌动蛋白为基础的细胞骨架，赋予BTB动力学。这篇及时的综述基于该领域的最新发现，对BTB的独特生物学和调控进行了更新，重点关注ABPs和非受体蛋白激酶的作用。
The blood-testis barrier (BTB) is an important ultrastructure in the testis since the onset of spermatogenesis coincides with the establishment of a functional barrier in rodents and humans. It is also noted that a delay in the assembly of a functional BTB following treatment of neonatal rats with drugs such as diethylstilbestrol or adjudin also delays the first wave of spermiation. While the BTB is one of the tightest blood-tissue barriers, it undergoes extensive remodeling, in particular at stage VIII of the epithelial cycle to facilitate the transport of preleptotene spermatocytes connected in clones across the immunological barrier. Without this timely transport of preleptotene spermatocytes derived from type B spermatogonia, meiosis will be arrested, causing aspermatogenesis. Yet the biology and regulation of the BTB remains largely unexplored since the morphological studies in the 1970s. Recent studies, however, have shed new light on the biology of the BTB. Herein, we critically evaluate some of these findings, illustrating that the Sertoli cell BTB is regulated by actin binding proteins (ABPs), likely supported by non-receptor protein kinases, to modulate the organization of actin microfilament bundles at the site. Furthermore, microtubule (MT)-based cytoskeleton is also working in concert with the actin-based cytoskeleton to confer BTB dynamics. This timely review provides an update on the unique biology and regulation of the BTB based on the latest findings in the field, focusing on the role of ABPs and non-receptor protein kinases.